Reactivity
Mouse, Human, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-DYQSKRTEGIV, from the C-Terminus of protein sequence according to NP_058590.1NP_848488.2.
Specificity
Mouse RAMP1. This antibody is expected to recognize isoform 1 (NP_058590.1) only.
Applications
- Western blot (0.3 - 1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:64000)
Usage
Peptide ELISA: antibody detection limit dilution 1:64000. Western Blot: experiments gave bands at approx. 18kD and 40kD in Rat Brain lysate after 0.3 ug/ml antibody staining. The appearance of a band with much higher molecular weight corresponds to earlier findings in literature with different antibodies (Hilairet, J Biol Chem. 2001 Nov 9;276(45):42182-90.; PMID: 11535606). This protein has a calculated MW of 16.9kD according to Mouse NP_058590.1 and 16.8kD according to Rat NP_113833.1. Recommended concentration: 0.3-1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee