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Antibodies > RAF1 / RAF > LS-C800926
 
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Catalog Number Size Price
LS-C800926-100 100 µl (1 mg/ml) $379 
LS-C800926-200 200 µl (1 mg/ml) $421 
RAF1 / RAF Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RAF1 / RAF Antibody - Western blot analysis of Phospho-C-RAF (Ser338) expression in various lysates
RAF1 / RAF Antibody - Western blot analysis of C-RAF phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RAF1 / RAF Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RAF1 / RAF Antibody - Western blot analysis of Phospho-C-RAF (Ser338) expression in various lysates
RAF1 / RAF Antibody - Western blot analysis of C-RAF phosphorylation expression in PMA treated NIH-3T3 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
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Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (phospho‑Ser338, IF, WB) LS‑C800926

Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (phospho‑Ser338, IF, WB) LS‑C800926

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Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (pSer338) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800926-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (pSer338) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RAF antibody LS-C800926 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) (pSer338) from human. It is reactive with human, mouse and rat. Validated for IF, Peptide-ELISA and WB.
Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human C-RAF around the phosphorylation site of Serine 338.
Epitope
pSer338
Specificity
Phospho-C-RAF (Ser338) Antibody detects endogenous levels of C-RAF only when phosphorylated at Serine 338.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

Publications (0)

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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6737 Mowry Ave
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