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Antibodies > RAF1 / RAF > LS-C800925
 
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Catalog Number Size Price
LS-C800925-100 100 µl (1 mg/ml) $379 
LS-C800925-200 200 µl (1 mg/ml) $421 
RAF1 / RAF Antibody - 1:200 staining human colon carcinoma sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RAF1 / RAF Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RAF1 / RAF Antibody - Western blot analysis of C-RAF phosphorylation expression in K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RAF1 / RAF Antibody - Western blot analysis of Phospho-C-RAF (Ser259) expression in various lysates
RAF1 / RAF Antibody - 1:200 staining human colon carcinoma sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22° C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
RAF1 / RAF Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
RAF1 / RAF Antibody - Western blot analysis of C-RAF phosphorylation expression in K562 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
RAF1 / RAF Antibody - Western blot analysis of Phospho-C-RAF (Ser259) expression in various lysates
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Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (phospho‑Ser259, IHC, IF, WB) LS‑C800925

Polyclonal Rabbit anti‑Human RAF1 / RAF Antibody (phospho‑Ser259, IHC, IF, WB) LS‑C800925

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Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (pSer259) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C800925-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
RAF1 / RAF Rabbit anti-Human Polyclonal (pSer259) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
RAF antibody LS-C800925 is an unconjugated rabbit polyclonal antibody to RAF (RAF1) (pSer259) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human RAF1 / RAF
Synonyms
RAF1 | C-Raf | C-raf-1 | CRAF | NS5 | Raf-1 | Proto-oncogene c-RAF | Oncogene RAF1 | RAF | Raf1 kinase
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human C-RAF around the phosphorylation site of Serine 259.
Epitope
pSer259
Specificity
Phospho-C-RAF (Ser259) Antibody detects endogenous levels of C-RAF only when phosphorylated at Serine 259.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About RAF1 / RAF
P04049 NM_002880 NP_002871.1

Publications (0)

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IHC‑plus™ RAF1 / RAF Antibody (N‑Terminus) LS‑B6239
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, ELISA
RAF1 / RAF Antibody - IHC of paraffin-embedded liver cancer tissues using RAF1 mouse monoclonal antibody with DAB staining.
RAF1 / RAF Antibody (clone 4G4) LS‑C169472
Species: Human
Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry, ELISA
RAF1 / RAF Antibody - Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.
RAF1 / RAF Antibody (aa1‑30) LS‑C97203
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Applications: IHC, Immunofluorescence, Western blot, Flow Cytometry
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Species: Human
Applications: IHC, Western blot, ELISA
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Species: Human, Monkey
Applications: Western blot, Immunoprecipitation
RAF1 / RAF Antibody - Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ RAF1 / RAF Antibody (C‑Terminus) LS‑B14835
Species: Human, Mouse, Rat, Bovine, Chicken
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
See all RAF1 / RAF Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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