Target
Human RAD54L
Synonyms
RAD54L | HR54 | HRAD54 | RAD54 (S.cerevisiae)-like | RAD54-like (S. cerevisiae) | RAD54 | RAD54 homolog | RAD54A | HHR54
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Affinity purified
Immunogen
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to a N-Terminal region near aa 1-25 of Human RAD54 protein.
Specificity
BLAST analysis indicates 100 % homology of the immunizing sequence with RAD54 from human. Cross reactivity with RAD54 protein homologues from other sources may not occur as sequence homology varies by at least one amino acid residue in this sequence.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:10000 - 1:40000)
Usage
This affinity purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 84 kD in size corresponding to RAD54 protein by western blotting in the appropriate cell lysate or extract. Splice variants exist for this protein that may result in the detection of lower molecular weight bands.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at 4°C or -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee