Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Purification
Immunoaffinity purified
Immunogen
Mouse IgG1 gamma 1 chain
Specificity
Recognizes mouse IgG1 heavy chain. Cross-reactivity against other mouse or human heavy or light chain isotypes is < 1%. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-fluorescein, anti-rabbit serum, mouse IgG and mouse Serum.
Applications
- Immunofluorescence (1:500 - 1:2500)
- Western blot
- Flow Cytometry (1:2000 - 1:10000)
- ELISA
Usage
Suitable for use in ELISA, Immunomicroscopy, Flow Cytometry, FACS analysis and fluorescent Western Blot. Also suitable for multiplex analysis. Immunomicroscopy: 1:500-1:2500. Flow Cytometry: 1:2000-1:10000. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized powder from PBS, pH 7.2, 10 mg/ml BSA (IgG, protease free), 0.15M sodium chloride, 0.01% sodium azide.
Reconstitution
Reconstitute with 1.0 ml sterile ddH2O
Storage
Store lyophilized powder -20°C. Reconstitute with sterile ddH20, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee