Target
Monkey IgG + IgA + IgM
Reactivity
Monkey
(tested or 100% immunogen sequence identity)
Conjugations
FITC.
Also available
Unconjugated or conjugated with
Biotin,
HRP.
Purification
Purified IgG
Immunogen
Purified polyclonal monkey IgG, and IgA and IgM containing fractions isolated from Rhesus monkey serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Monkey IgG, IgA and IgM, heavy and light chains.
Applications
- IHC
- IHC - Frozen
- ICC
- Immunofluorescence
- ELISA
- Applications tested for the base form of this product only
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
The defined antibody specificity is directed to the major isotypes of the monkey immunoglobulin system and to the surface determinants of the common Fab portion as tested against as tested in immunoelectrophoresis and double radial immunodiffusion (Ouchterlony). Direct immunofluorescence staining of cytoplasmic Ig of appropriately treated cell and tissue substrates. to demonstrate immunoglobulins or specific antibodies in cells and tissues. to identify circulating antibodies in serodiagnostic microbiology and autoimmune diseases. to identify a specific antigen or immune complex using a reference antibody of monkey origin in the middle layer of the indirect test procedure. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee