Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Affinity chromatography
Immunogen
Human IgM Fc5µ fragment
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Fluorescein, anti-Rabbit Serum, Human IgM and Human Serum. No reaction was observed against Human IgG or Human IgA. Specificity was confirmed by ELISA minimal cross reactivity against other human heavy or light chain isotypes.
Applications
- Immunofluorescence (1:1000 - 1:5000)
- Flow Cytometry (1:500 - 1:2500)
- Fluorophore-Linked Immunosorbent Assay (1:10000 - 1:50000)
Usage
Suitable for immunomicroscopy and flow cytometry or FACS analysis as well as other antibody based fluorescent assays requiring lot-to-lot consistency. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 1 mL of deionized water.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee