Reactivity
Sheep
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
FITC,
HRP.
Immunogen
Highly purified normal IgM isolated from pooled sheep serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Sheep IgM, Fc specific.
Usage
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal sheep serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in sheep serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of sheep IgM from serum or plasma.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee