Reactivity
Mouse
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
Biotin,
FITC,
HRP,
TRITC.
Immunogen
Highly purified homogenous IgM isolated from mouse serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Mouse IgM, Fc specific.
Usage
The reactivity of the antiserum is restricted to the Fc part of the IgM molecule. In immunoelectrophoresis and radial immunodiffusion, using various antiserum concentrations against normal mouse serum a single precipitin line is obtained which shows a reaction of identity with the precipitin line obtained with purified IgM. No precipitation reaction is obtained with purified IgG, IgA, and IgG/Fab fragments. In precipitating techniques as immunoelectrophoresis and radial immunodiffusion to identify the presence of IgM in mouse serum and other body fluids or to determine its concentration. To prepare an immunoadsorbent for the purification of mouse IgM from serum or plasma. This antiserum is not intended for use in non-precipitating antibody binding or other highly sensitive assays. For such use labeled and unlabelled cytochemical and immunoassay grade reagents meeting the necessary additional specificity and performance requirements are available.
Presentation
Delipidated, heat-inactivated, lyophilized antiserum. No preservative.
Reconstitution
1 ml Distilled water
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee