Rabbit Pyruvate Kinase

Goat

Rabbit (tested or 100% immunogen sequence identity)

IgG Polyclonal

Horseradish Peroxidase

Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.

Lyophilized

Pyruvate Kinase [Rabbit Muscle].

Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. This product has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other tissues and species may occur but have not been specifically determined.

• Western blot (1:500 - 1:2000)
• ELISA (1:4000 - 1:20000)

Anti-Pyruvate Kinase has been assayed against 1.0 ug of Pyruvate Kinase [Rabbit Muscle] in a standard capture ELISA using ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:15000 to 1:60000 of the reconstitution concentration is suggested for this product. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.

Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide

Reconstitute in 100 µL of deionized water.

Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.