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Catalog Number Size Price
LS-C209693-100 100 µg (1 mg/ml) $552 
Pyruvate Kinase Antibody - Western Blot of Goat Anti-Pyruvate Kinase Biotin Conjugate. Lane 1: Opal Prestain Molecular Weight Marker. Lane 2: 50ng of Pyruvate Kinase. Primary Antibody: Goat Anti-Pyruvate Kinase Biotin at 1ug/mL o/n @ 4C. Secondary Antibody: Streptavidin-HRP: Sat 1:40,000 for 30 min at RT.

Polyclonal Goat anti‑Rabbit Pyruvate Kinase Antibody (Lyophilized, Biotin, WB) LS‑C209693

Polyclonal Goat anti‑Rabbit Pyruvate Kinase Antibody (Lyophilized, Biotin, WB) LS‑C209693

Antibody:
Pyruvate Kinase Goat anti-Rabbit Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Rabbit
Format:
Biotin, Lyophilized
Price
Catalog Number
$552
LS-C209693-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Pyruvate Kinase Goat anti-Rabbit Polyclonal (Lyophilized) (Biotin) Antibody
Application:
WB, ELISA
Reactivity:
Rabbit
Format:
Biotin, Lyophilized

Specifications

Description
Pyruvate Kinase antibody LS-C209693 is a biotin-conjugated goat polyclonal antibody to rabbit Pyruvate Kinase. Validated for ELISA and WB.
Target
Rabbit Pyruvate Kinase
Host
Goat
Reactivity
Rabbit (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Biotin
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Pyruvate Kinase [Rabbit Muscle].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Biotin, anti-Goat Serum as well as purified and partially purified Pyruvate Kinase [Rabbit Muscle]. Anti-Pyruvate Kinase has been reported to react with all forms of pyruvate kinase (pan M-PK). Cross reactivity against Pyruvate Kinase from other sources may occur but have not been specifically determined.
Applications
  • Western blot (1:200 - 1:1000)
  • ELISA (1:1000 - 1:5000)
Usage
Anti-Pyruvate Kinase Antibody has been assayed against 1.0 ug of Pyruvate Kinase in a standard capture ELISA using Peroxidase Conjugated Streptavidin ABTS as a substrate for 30 minutes at room temperature. A working dilution of 1:3000 to 1:15000 of the reconstitution concentration is suggested for Anti-Pyruvate Kinase Antibody. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 12/22/2024