Chicken PXN / Paxillin

Mouse

Chicken, Human, Mouse, Rat, Dog (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

Unconjugated

Purified

Unmodified

Affinity purified phosphotyrosine-containing proteins from Rous sarcoma virus transformed chick embryo fibroblasts.

Reacts strongly with two bands on Western blots of total cell lysate from chick embryo fibroblasts or rat brain. The lower molecular band is believed to be either an isoform or a degradation product of the 68kD protein, since it is found also on SDS-PAGE of affinity purified paxillin. Recognizes human, canine, mouse, chick and rat.

• IHC
• ICC
• Western blot (1:1000 - 1:10000)
• Immunoprecipitation
• ELISA

Western blot: 1:1000-1:10000. ELISA. Immunohistochemistry. Immunocytochemistry. Immunoprecipitation. Control Lysate: A431 Lysate: Prepared from a human epidermoid carcinoma cell line. Cell lysate is available to be used as a positive control in Western blotting. Cell lysate is provided at a concentration of 1 ug/ml in SDS-PAGE buffer (62 mM Tris pH 6.8, 2% SDS, 0.9% beta mercaptoethanol, 0.003% bromophenol blue and 5% glycerol). 5-10 ul/lane is sufficient for Western blot.

50% glycerol, 20 mM sodium phosphate, pH 7.5, 150 mM sodium chloride, 1.5 mM sodium azide.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P49023 NCBI: NM_002859 NP_002850.2