Target
Human PTCH1 / Patched 1
Synonyms
PTCH1 | NBCCS | Patched 1 | Patched homolog 1 | Protein patched homolog 1 | PTC1 | PTCH | PTCH protein +12b | PTCH protein -10 | PTCH protein -3,4,5 | Patched | Patched protein homolog 1 | BCNS | HPE7 | Patched homolog | PTC | PTCH protein +4 | PTCH11
Reactivity
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit, Sheep
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-EELKKINYQVDSWEE, from the internal region of the protein sequence according to NP_000255.2NP_001077072.1NP_001077071.1NP_001077073.1.
Specificity
Human PTCH1 / Patched. This antibody is expected to recognize all reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1
Applications
- Immunofluorescence (5 µg/ml)
- Western blot (1 - 3 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Mouse fetal Kidney lysates (calculated MW of 159kD according to NP_032983.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee