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Catalog Number Size Price
LS-C186591-100 100 µg (0.5 mg/ml) $503 
PTCH1 / Patched 1 Antibody - PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
PTCH1 / Patched 1 Antibody - PTCH1 antibody (2 ug/ml) staining of Mouse fetal Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
PTCH1 / Patched 1 Antibody - PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic and Golgi apparatus staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (4ug/ml).
PTCH1 / Patched 1 Antibody - PTCH1 antibody (2 ug/ml) staining of Mouse fetal Kidney lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
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Polyclonal Goat anti‑Human PTCH1 / Patched 1 Antibody (aa266‑280, IF, WB) LS‑C186591

Polyclonal Goat anti‑Human PTCH1 / Patched 1 Antibody (aa266‑280, IF, WB) LS‑C186591

Antibody:
PTCH1 / Patched 1 Goat anti-Human Polyclonal (aa266-280) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified
Price
Catalog Number
$503
LS-C186591-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PTCH1 / Patched 1 Goat anti-Human Polyclonal (aa266-280) Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit, Sheep
Format:
Unconjugated, Unmodified

Specifications

Description
Patched 1 antibody LS-C186591 is an unconjugated goat polyclonal antibody to Patched 1 (PTCH1) (aa266-280) from human. It is reactive with human, mouse, rat and other species. Validated for IF, Peptide-ELISA and WB.
Target
Human PTCH1 / Patched 1
Synonyms
PTCH1 | NBCCS | Patched 1 | Patched homolog 1 | Protein patched homolog 1 | PTC1 | PTCH | PTCH protein +12b | PTCH protein -10 | PTCH protein -3,4,5 | Patched | Patched protein homolog 1 | BCNS | HPE7 | Patched homolog | PTC | PTCH protein +4 | PTCH11
Host
Goat
Reactivity
Human, Monkey, Mouse, Rat, Dog, Horse, Pig, Rabbit, Sheep (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence C-EELKKINYQVDSWEE, from the internal region of the protein sequence according to NP_000255.2NP_001077072.1NP_001077071.1NP_001077073.1.
Epitope
aa266-280
Specificity
Human PTCH1 / Patched. This antibody is expected to recognize all reported isoforms (NP_000255.2; NP_001077072.1; NP_001077071.1; NP_001077073.1). Reported variants represent identical protein: NP_001077075.1, NP_001077074.1, NP_001077073.1, NP_001077076.1
Applications
  • Immunofluorescence (5 µg/ml)
  • Western blot (1 - 3 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western blot: Approx 140kD band observed in Mouse fetal Kidney lysates (calculated MW of 159kD according to NP_032983.1). Recommended concentration: 1-3 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTCH1 / Patched 1
Q13635 NM_000264 NP_000255.2

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/22/2024