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Catalog Number Size Price
LS-B3939-50 50 µg (0.5 mg/ml) $485 
PTCH1 / Patched 1 Antibody - Anti-PTCH1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PTCH1 / Patched 1 Antibody - Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PTCH1 / Patched 1 Antibody - PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PTCH1 / Patched 1 Antibody - PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PTCH1 / Patched 1 Antibody - Anti-PTCH1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PTCH1 / Patched 1 Antibody - Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
PTCH1 / Patched 1 Antibody - PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed NIH3T3 cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml), showing Golgi/membrane staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
PTCH1 / Patched 1 Antibody - PTCH1 / Patched 1 antibody immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (5ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
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IHC‑plus™ Polyclonal Goat anti‑Human PTCH1 / Patched 1 Antibody (aa1431‑1442, IHC, IF) LS‑B3939

IHC‑plus™ Polyclonal Goat anti‑Human PTCH1 / Patched 1 Antibody (aa1431‑1442, IHC, IF) LS‑B3939

Note: This antibody replaces LS-C55338
Antibody:
PTCH1 / Patched 1 Goat anti-Human Polyclonal (aa1431-1442) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Monkey, Bat, Dog, Horse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$485
LS-B3939-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PTCH1 / Patched 1 Goat anti-Human Polyclonal (aa1431-1442) Antibody
Application:
IHC, IHC-P, IF, Peptide-ELISA
Reactivity:
Human, Monkey, Bat, Dog, Horse
Format:
Unconjugated, Unmodified

Specifications

Description
Patched 1 antibody LS-B3939 is an unconjugated goat polyclonal antibody to Patched 1 (PTCH1) (aa1431-1442) from human. It is reactive with human, bat, dog and other species. Validated for IF, IHC and Peptide-ELISA. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PTCH1 / Patched 1
Synonyms
PTCH1 | NBCCS | Patched 1 | Patched homolog 1 | Protein patched homolog 1 | PTC1 | PTCH | PTCH protein +12b | PTCH protein -10 | PTCH protein -3,4,5 | Patched | Patched protein homolog 1 | BCNS | HPE7 | Patched homolog | PTC | PTCH protein +4 | PTCH11
Host
Goat
Reactivity
Human, Monkey, Bat, Dog, Horse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Modifications
Unmodified
Immunogen
Peptide with sequence ELQDVECEERPR, from the C-Terminus of protein sequence according to NP_000255.1NP_001077072.1NP_001077071.1NP_001077073.1NP_001077074.1NP_001077075.1NP_001077076.1.
Epitope
aa1431-1442
Specificity
Human PTCH1. This antibody is expected to recognise all reported isoforms. Variants (NP_001077073.1; NP_001077074.1; NP_001077075.1; NP_001077076.1) encode the same isoform.
Applications
  • IHC
  • IHC - Paraffin (3.75 µg/ml)
  • Immunofluorescence (5 µg/ml)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:8000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PTCH1 / Patched 1
Q13635 NM_000264 NP_000255.2

Validation

PTCH1 / Patched 1 Antibody - Anti-PTCH1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PTCH1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PTCH1 antibody IHC of human colon. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

PTCH1 / Patched 1 Antibody - Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.
Anti-PTCH1 antibody IHC of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 3.75 ug/ml.

LSBio Ratings

IHC-plus™ PTCH1 / Patched 1 Antibody (aa1431-1442) for IHC, IF/Immunofluorescence LS-B3939 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)

Publications (1)

Ptch2 loss drives myeloproliferation and myeloproliferative neoplasm progression. Klein C, Zwick A, Kissel S, Forster CU, Pfeifer D, Follo M, Illert AL, Decker S, Benkler T, Pahl H, Oostendorp RA, Aumann K, Duyster J, Dierks C. The Journal of experimental medicine. 2016 213:273-90. (WB, ELISA; Human)

Customer Reviews (0)


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Requested From: United States
Date Requested: 11/22/2024