Target
Mouse Proenkephalin / PENK
Synonyms
PENK | Enkephalin A | Met enkephalin | Preproenkephalin | Proenkephalin | Proenkephalin-A
Reactivity
Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-YKDSSKQDESH, from the internal region of the protein sequence according to NP_001002927.1.
Applications
- IHC - Frozen (0.02 - 0.05 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:32000)
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Usage
Peptide ELISA: antibody detection limit dilution 1:32000. Western Blot: Preliminary experiments gave an approx 40kD band in Rat Brain lysates after 0.2 ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 30.8kD according to Mouse NP_001002927.1 and 30.9kD according to Rat NP_058835.1. The 40kD band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies / lysates? Have any further splice variants / modified forms been reported?
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About Proenkephalin / PENK