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Catalog Number	Size	Price
LS-C354069-100	100 µl (1 mg/ml)	$299

PRKCZ / PKC-Zeta Antibody - Immunohistochemical analysis of PKC zeta staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.

PRKCZ / PKC-Zeta Antibody - Immunofluorescent analysis of PKC zeta staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

PRKCZ / PKC-Zeta Antibody - Western blot analysis of PKC zeta expression in HeLa (A); NIH3T3 (B); PC12 (C); rat kidney (D); rat heart (E) whole cell lysates.

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Polyclonal Rabbit anti‑Human PRKCZ / PKC‑Zeta Antibody (C‑Terminus, IHC, IF, WB) LS‑C354069

Name: Anti-PRKCZ / PKC-Zeta Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C354069-100
Price: 299.0000 USD
Availability: InStock

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Antibody:

PRKCZ / PKC-Zeta Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Monkey, Mouse, Rat, Rabbit, Zebrafish

Format:

Unconjugated, Unmodified

$299

LS-C354069-100

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Overview

Antibody:

PRKCZ / PKC-Zeta Rabbit anti-Human Polyclonal (C-Terminus) Antibody

Application:

IHC, IHC-P, ICC, IF, WB, IP

Reactivity:

Human, Monkey, Mouse, Rat, Rabbit, Zebrafish

Format:

Unconjugated, Unmodified

Specifications

Description

PKC-Zeta antibody LS-C354069 is an unconjugated rabbit polyclonal antibody to PKC-Zeta (PRKCZ) (C-Terminus) from human. It is reactive with human, mouse, rat and other species. Validated for ICC, IF, IHC, IP and WB.

Target

Human PRKCZ / PKC-Zeta

Synonyms

See All PRKCZ / PKC-Zeta Antibodies

Host

Rabbit

Reactivity

Human, Monkey, Mouse, Rat, Rabbit, Zebrafish (tested or 100% immunogen sequence identity)

Clonality

Polyclonal

Conjugations

Unconjugated

Purification

Immunoaffinity purified

Modifications

Unmodified

Immunogen

KLH-conjugated synthetic peptide encompassing a sequence within the C-Terminus of human PKC zeta.

Epitope

C-Terminus

Specificity

Recognizes endogenous levels of PKC zeta protein.

Applications

IHC
IHC - Paraffin (1:100 - 1:200)
ICC (1:100 - 1:500)
Immunofluorescence (1:100 - 1:500)
Western blot (1:500 - 1:1000)
Immunoprecipitation (1:10 - 1:100)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Presentation

PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol

Storage

Store at -20°C. Aliquot to avoid freeze/thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

LSBio Guarantee

About PRKCZ / PKC-Zeta

Uniprot: Q05513 NCBI: NM_002744 NP_002735.3