Target
Human PRKCH / PKC-Eta
Synonyms
PRKCH | PKCL | Protein kinase c eta | Protein kinase C, eta | PKC-L | PRKCL | NPKC-eta | PKC eta | Protein kinase C eta type
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phosphopeptide derived from a region of human PKC that contains threonine 655. The sequence is conserved in mouse and rat.
Specificity
Reactivity: Human PKC. Mouse and rat (100% homologous) PKC have not been tested, but are expected to react. This antibody did not cross-react with any other PKC isoforms tested.
Usage
Suitable for use in Western blotting applications. For Western blotting applications, we recommend using the antibody at 0.35-1.0 ug/ml. At 0.50 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: Jurkat cells treated with PMA, a phorbol ester.
Presentation
Supplied as liquid in PBS (without Mg2+, Ca2+), pH 7.3, 1 mg/ml BSA (IgG, protease free), 0.05% sodium azide
Storage
Short term: store at 4°C. Long term: aliquot and store at -20°C. Avoid freeze-thaw cycles. Store undiluted.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee