Target
Human PRKCG / PKC-Gamma
Synonyms
PRKCG | PKC-gamma | PRKC-gamma | Protein kinase C gamma | Protein kinase C gamma type | PKC Gamma | PKCC | PKCG | Pkcgamma | Protein kinase C, gamma | SCA14
Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic phosphopeptide derived from a region of human PKC that contains threonine 514.
Specificity
Reactivity: Human PKC. Mouse and rat (88% homologous) PKC have not been tested, but are expected to react. PKC,, (75%), I, II, and (63%) may cross-react in cells expressing high levels of these proteins.
Usage
Suitable for use in Western blotting applications. For Western blotting applications, we recommend using the antibody at 0.5-1.0 ug/ml. At 0.50 ug/ml, the dilution provides 100 ml working solution, which at 10 ml/blot allows 10 blots to be performed. Positive control: Hela cells treated with PMA, a phorbol ester.
Presentation
PBS (without Mg2+, Ca2+), 1 mg/ml BSA (IgG, protease free), pH 7.2, 0.1% sodium azide.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee