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Antibodies > PRKCD / PKC-Delta > LS-C801157
 
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Catalog Number Size Price
LS-C801157-100 100 µl (1 mg/ml) $379 
LS-C801157-200 200 µl (1 mg/ml) $421 
PRKCD / PKC-Delta Antibody - 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
PRKCD / PKC-Delta Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKCD / PKC-Delta Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCD / PKC-Delta Antibody - Western blot analysis of PKC delta phosphorylation expression in HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCD / PKC-Delta Antibody - Western blot analysis of Phospho-PKC delta (Tyr313) expression in various lysates
PRKCD / PKC-Delta Antibody - 1/100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.
PRKCD / PKC-Delta Antibody - 1:100 staining human breast carcinoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PRKCD / PKC-Delta Antibody - Staining NIH-3T3 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PRKCD / PKC-Delta Antibody - Western blot analysis of PKC delta phosphorylation expression in HepG2 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PRKCD / PKC-Delta Antibody - Western blot analysis of Phospho-PKC delta (Tyr313) expression in various lysates
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Polyclonal Rabbit anti‑Human PRKCD / PKC‑Delta Antibody (phospho‑Tyr313, IHC, IF, WB) LS‑C801157

Polyclonal Rabbit anti‑Human PRKCD / PKC‑Delta Antibody (phospho‑Tyr313, IHC, IF, WB) LS‑C801157

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Antibody:
PRKCD / PKC-Delta Rabbit anti-Human Polyclonal (pTyr313) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801157-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PRKCD / PKC-Delta Rabbit anti-Human Polyclonal (pTyr313) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
PKC-Delta antibody LS-C801157 is an unconjugated rabbit polyclonal antibody to PKC-Delta (PRKCD) (pTyr313) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PRKCD / PKC-Delta
Synonyms
PRKCD | MAY1 | NPKC-delta | PKCD | Protein kinase C delta type | Protein kinase C delta VIII | Protein kinase C, delta | Pkcdelta | PKC delta | Protein kinase c delta | Tyrosine-protein kinase PRKCD
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human PKC delta around the phosphorylation site of Tyrosine 313.
Epitope
pTyr313
Specificity
Phospho-PKC delta (Tyr313) Antibody detects endogenous levels of PKC delta only when phosphorylated at Tyrosine 313.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PRKCD / PKC-Delta
Q05655 NM_006254 NP_006245.2

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Applications: IHC, IHC - Frozen, ICC, Western blot, ELISA
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PRKCD / PKC‑Delta Antibody (Biotin) LS‑C305829
Species: Human
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PRKCD / PKC-Delta Antibody - Western blot analysis of PKC delta (pY52) expression in MCF7 serum starvation-treated (A); HeLa PMA-treated (B); Jurkat (C); Raw264.7 serum starvation-treated (D); H9C2 serum starvation-treated (E) whole cell lysates.
PRKCD / PKC‑Delta Antibody (phospho‑Tyr52) LS‑C352714
Species: Human, Monkey, Mouse, Rat, Bovine, Dog, Pig, Rabbit, Chicken
Applications: Western blot
PRKCD / PKC-Delta Antibody - Anti-PRKCD / PKC-Delta antibody IHC staining of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:50.
IHC‑plus™ PRKCD / PKC‑Delta Antibody (aa30‑79) LS‑B11341
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PRKCD / PKC-Delta Antibody - Immunohistochemistry of paraffin-embedded Human thyroid cancer using PRKCD Polyclonal Antibody at dilution of 1:30.
PRKCD / PKC‑Delta Antibody LS‑C404622
Species: Human, Mouse, Rat
Applications: IHC, Western blot, ELISA
See all PRKCD / PKC-Delta Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/7/2025
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