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Catalog Number Size Price
LS-C774030-100 100 µg (1 mg/ml) $442 
PPP5C Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody  at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.
PPP5C Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.
PPP5C Antibody - Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 µg). Lane 3: Human HEK293 (15 µg). Lane 4: Human Jurkat (15 µg). Load: 15 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody  at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.
PPP5C Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 2% Formaldehyde for 20 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody  at 1:50 for 1 hour at RT. Secondary Antibody: Alexa Fluor 488 Goat Anti-Mouse (green) at 1:100 for 1 hour at RT. Counterstain: DAPI (blue) nuclear stain. Magnification: 63x.
PPP5C Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Tissue: Cervical cancer cell line (HeLa). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody  at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:100 for 60 min at RT. Counterstain: DAPI (blue) nuclear stain at 1:5000 for 5 min RT. Localization: Nucleus, Cytoplasm. Magnification: 40X.
PPP5C Antibody - Western Blot analysis of Human A431, HEK293, and Jurkat cell lysates showing detection of ~58 kDa PP5 protein using Mouse Anti-PP5 Monoclonal Antibody, Clone 12F7. Lane 1: MW Ladder. Lane 2: Human A431 (15 µg). Lane 3: Human HEK293 (15 µg). Lane 4: Human Jurkat (15 µg). Load: 15 µg. Block: 5% Skim Milk for 1 hour at RT. Primary Antibody: Mouse Anti-PP5 Monoclonal Antibody  at 1:500 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:200 for 1 hour at RT. Color Development: ECL solution for 6 min at RT. Predicted/Observed Size: ~58 kDa.
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Monoclonal Mouse anti‑Human PPP5C Antibody (clone 12F7, IF, WB) LS‑C774030

Monoclonal Mouse anti‑Human PPP5C Antibody (clone 12F7, IF, WB) LS‑C774030

Antibody:
PPP5C Mouse anti-Human Monoclonal (12F7) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$442
LS-C774030-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PPP5C Mouse anti-Human Monoclonal (12F7) Antibody
Application:
IF, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
PPP5C antibody LS-C774030 is an unconjugated mouse monoclonal antibody to human PPP5C. Validated for IF and WB.
Target
Human PPP5C
Synonyms
PPP5C | PP-T | Protein phosphatase T | PPP5 | PPT | Protein phosphatase 5 | PP5
Host
Mouse
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG1 Monoclonal
Clone
12F7
Conjugations
Unconjugated
Purification
Protein G purified
Modifications
Unmodified
Immunogen
Full length human PP5 protein
Specificity
Detects ~58 kDa.
Applications
  • Immunofluorescence (1:100)
  • Western blot (1:1000)
Usage
Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP5C
P53041 NM_006247 NP_006238.1

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Requested From: United States
Date Requested: 11/22/2024