Target
Human PPP3CB
Synonyms
PPP3CB | Calcineurin A2 | CALNB | CALNA2 | CNA2 | Calcineurin A beta | Cna beta | PP2Bbeta | Calcineurin b | CAM-PRP catalytic subunit | Phosphatase 2b beta | pp2b-beta
Reactivity
Human, Monkey, Mouse, Rat, Bat, Bovine, Dog, Horse, Pig, Xenopus
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
A 20 residue synthetic peptide EDFGNEKSQEHFSHNTVRGC based on the human calcineurin A (aa 264-283) was synthesized and the peptide coupled to KLH. This sequence is identical to rat and mouse calcineurin A over these residues. Percent identity by BLAST analysis: Human, Gorilla, Gibbon, Monkey, Marmoset, Mouse, Rat, Elephant, Panda, Dog, Bovine, Bat, Horse, Pig, Opossum, Platypus, Xenopus (100%); Stickleback, Zebrafish (95%); Orangutan, Hamster, Turkey (90%).
Specificity
Recognizes an ~61kD protein, corresponding to the apparent molecular mass of recombinant calcineurin A. Species cross-reactivity: Human, mouse, rat, rabbit, canine, Drosophila, hamster, bovine, chicken, monkey, porcine and sheep.
Applications
- Western blot (2 µg/ml)
- Immunoprecipitation (10 µg/ml)
Usage
Suitable for use in Western Blot and Immunoprecipitation. Western Blot (ECL): 0.5 ug/ml. Immunoprecipitation: 10 ug/ml. Positive control: Rat Brain Tissue Extract.
Presentation
25 mM Sodium Borate, BBS, pH 8.4, 75 mM NaCl, 5 mM EDTA, 100 mM Boric acid
Storage
Short term: 4°C; Long term: Add glycerol (40-50%) -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee