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Antibodies > PPP1R14A / CPI-17 > LS-C801222
 
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Catalog Number Size Price
LS-C801222-100 100 µl (1 mg/ml) $379 
LS-C801222-200 200 µl (1 mg/ml) $421 
PPP1R14A / CPI-17 Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PPP1R14A / CPI-17 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PPP1R14A / CPI-17 Antibody - Western blot analysis of CPI17 alpha phosphorylation expression in RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PPP1R14A / CPI-17 Antibody - Western blot analysis of Phospho-CPI17 alpha (Thr38) expression in various lysates
PPP1R14A / CPI-17 Antibody - 1:100 staining human brain tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.
PPP1R14A / CPI-17 Antibody - Staining HT29 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
PPP1R14A / CPI-17 Antibody - Western blot analysis of CPI17 alpha phosphorylation expression in RAW264.7 whole cells lysates. The lane on the left is treated with the antigen-specific peptide.
PPP1R14A / CPI-17 Antibody - Western blot analysis of Phospho-CPI17 alpha (Thr38) expression in various lysates
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Polyclonal Rabbit anti‑Human PPP1R14A / CPI‑17 Antibody (phospho‑Thr38, IHC, IF, WB) LS‑C801222

Polyclonal Rabbit anti‑Human PPP1R14A / CPI‑17 Antibody (phospho‑Thr38, IHC, IF, WB) LS‑C801222

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Antibody:
PPP1R14A / CPI-17 Rabbit anti-Human Polyclonal (pThr38) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C801222-100
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PPP1R14A / CPI-17 Rabbit anti-Human Polyclonal (pThr38) Antibody
Application:
IHC, IF, WB, Peptide-ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
CPI-17 antibody LS-C801222 is an unconjugated rabbit polyclonal antibody to CPI-17 (PPP1R14A) (pThr38) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.
Target
Human PPP1R14A / CPI-17
Synonyms
PPP1R14A | 17-KDa protein | CPI-17 | PPP1INL | CPI17
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human CPI17 alpha around the phosphorylation site of Threonine 38.
Epitope
pThr38
Specificity
Phospho-CPI17 alpha (Thr38) Antibody detects endogenous levels of CPI17 alpha only when phosphorylated at Threonine 38.
Applications
  • IHC (1:50 - 1:200)
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:500 - 1:2000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP1R14A / CPI-17
Q96A00 NP_150281.1

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Featured Products

PPP1R14A / CPI-17 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using CPI17 alpha (Phospho-Thr38) Antibody. The picture on the right is blocked with the phospho peptide.
PPP1R14A / CPI‑17 Antibody (phospho‑Thr38) LS‑C117328
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PPP1R14A / CPI-17 Antibody - Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using CPI17 alpha Antibody. The picture on the right is blocked with the synthesized peptide.
PPP1R14A / CPI‑17 Antibody (aa5‑54) LS‑C118085
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Immunofluorescence, Western blot, Peptide Enzyme-Linked Immunosorbent Assay
PPP1R14A / CPI-17 Antibody - Immunohistochemical analysis of CPI17 staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PPP1R14A / CPI‑17 Antibody (N‑Terminus) LS‑C368974
Species: Human, Mouse, Rat, Pig
Applications: IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot
PPP1R14A / CPI-17 Antibody - Western blot analysis of extracts of various cell lines, using PPP1R14A antibody at 1:1000 dilution. The secondary antibody used was an HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates were loaded 25ug per lane and 3% nonfat dry milk in TBST was used for blocking. An ECL Kit was used for detection and the exposure time was 60s.
PPP1R14A / CPI‑17 Antibody LS‑C496871
Species: Human, Mouse, Rat
Applications: Western blot
EPO / Erythropoietin Protein - Recombinant Erythropoietin By SDS-PAGE
Human EPO / Erythropoietin Protein (Recombinant His-GST) (aa28-193) - LS-G11249
Source: E. coli
Tag: His-GST
See all PPP1R14A / CPI-17 Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 11/12/2024
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