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Catalog Number Size Price
LS-C662237-10 10 µg $318 
LS-C662237-100 100 µg $470 
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Western blot analysis of CPI17 alpha using anti-CPI17 alpha antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: PANC-1 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPI17 alpha antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CPI17 alpha at approximately 20KD. The expected band size for CPI17 alpha is at 20KD.
PPP1R14A / CPI-17 Antibody - Western blot - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Flow Cytometry analysis of U20S cells using anti-CPI17 alpha antibody. Overlay histogram showing U20S cells stained with anti-CPI17 alpha antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPI17 alpha Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Immunohistochemistry - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Western blot analysis of CPI17 alpha using anti-CPI17 alpha antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat brain tissue lysates, Lane 2: mouse brain tissue lysates, Lane 3: PANC-1 whole Cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CPI17 alpha antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CPI17 alpha at approximately 20KD. The expected band size for CPI17 alpha is at 20KD.
PPP1R14A / CPI-17 Antibody - Western blot - Anti-CPI17 alpha Picoband Antibody
PPP1R14A / CPI-17 Antibody - Flow Cytometry analysis of U20S cells using anti-CPI17 alpha antibody. Overlay histogram showing U20S cells stained with anti-CPI17 alpha antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CPI17 alpha Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human PPP1R14A / CPI‑17 Antibody (IHC, WB) LS‑C662237

Polyclonal Rabbit anti‑Human PPP1R14A / CPI‑17 Antibody (IHC, WB) LS‑C662237

Antibody:
PPP1R14A / CPI-17 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C662237-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PPP1R14A / CPI-17 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, WB
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
CPI-17 antibody LS-C662237 is an unconjugated rabbit polyclonal antibody to human CPI-17 (PPP1R14A). Validated for IHC and WB.
Target
Human PPP1R14A / CPI-17
Synonyms
PPP1R14A | 17-KDa protein | CPI-17 | PPP1INL | CPI17
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human CPI17 alpha recombinant protein (Position: L30-Q126). Human CPI17 alpha shares 83.5% and 84.5% amino acid (aa) sequence identity with mouse and rat CPI17 alpha, respectively.
Specificity
Isoform 1 is detected in aorta and testis. Isoform 2 is detected in aorta. .
Applications
  • IHC
  • IHC - Paraffin
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPP1R14A / CPI-17
Q96A00 NP_150281.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/12/2024