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Antibodies > PPIA / Cyclophilin A > LS-C661866
 
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Catalog Number Size Price
LS-C661866-10 10 µg $318 
LS-C661866-100 100 µg $470 
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - Immunohistochemistry - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - Immunohistochemistry - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - Immunohistochemistry - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - IF analysis of Cyclophilin A using anti-Cyclophilin A antibody Cyclophilin A was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
PPIA / Cyclophilin A Antibody - Western blot - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - Flow Cytometry analysis of K562 cells using anti-PPIA antibody. Overlay histogram showing K562 cells stained with anti-PPIA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PPIA / Cyclophilin A Antibody - Flow Cytometry analysis of U937 cells using anti-PPIA antibody. Overlay histogram showing U937 cells stained with anti-PPIA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PPIA / Cyclophilin A Antibody - Flow Cytometry analysis of THP-1 cells using anti-PPIA antibody. Overlay histogram showing THP-1 cells stained with anti-PPIA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - IHC analysis of Cyclophilin A using anti-Cyclophilin A antibody. Cyclophilin A was detected in immunocytochemical section of THP-1 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/ml rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Avidin was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # SA1094) with DAB as the chromogen.
PPIA / Cyclophilin A Antibody - Immunohistochemistry - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - Immunohistochemistry - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - Immunohistochemistry - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - IF analysis of Cyclophilin A using anti-Cyclophilin A antibody Cyclophilin A was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2µg/mL rabbit anti-Cyclophilin A Antibody overnight at 4°C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37°C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.
PPIA / Cyclophilin A Antibody - Western blot - Anti-Cyclophilin A Picoband Antibody
PPIA / Cyclophilin A Antibody - Flow Cytometry analysis of K562 cells using anti-PPIA antibody. Overlay histogram showing K562 cells stained with anti-PPIA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PPIA / Cyclophilin A Antibody - Flow Cytometry analysis of U937 cells using anti-PPIA antibody. Overlay histogram showing U937 cells stained with anti-PPIA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
PPIA / Cyclophilin A Antibody - Flow Cytometry analysis of THP-1 cells using anti-PPIA antibody. Overlay histogram showing THP-1 cells stained with anti-PPIA antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PPIA Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human PPIA / Cyclophilin A Antibody (IHC, WB) LS‑C661866

Polyclonal Rabbit anti‑Human PPIA / Cyclophilin A Antibody (IHC, WB) LS‑C661866

Submit Review LSBio Guarantee
Antibody:
PPIA / Cyclophilin A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661866-10
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PPIA / Cyclophilin A Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, WB, Flo
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
Cyclophilin A antibody LS-C661866 is an unconjugated rabbit polyclonal antibody to human Cyclophilin A (PPIA). Validated for Flow, ICC, IHC and WB.
Target
Human PPIA / Cyclophilin A
Synonyms
PPIA | Cyclophilin | Cyclosporin A-binding protein | Cyclophilin A | PPIase A | Rotamase A | CYPH | T cell cyclophilin
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E.coli-derived human Cyclophilin A recombinant protein (Position: T116-E165). Human Cyclophilin A shares 98% and 95.9% amino acid (aa) sequence identity with mouse and rat Cyclophilin A, respectively.
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
  • Flow Cytometry
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PPIA / Cyclophilin A
P62937 NM_021130 NP_066953.1

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Featured Products

PPIA / Cyclophilin A Antibody - Western Blot analysis of PPIA expression in transfected 293T cell line by PPIA monoclonal antibody (M01), clone 1F4-1B5.Lane 1: PPIA transfected lysate(18 KDa).Lane 2: Non-transfected lysate.
PPIA / Cyclophilin A Antibody (clone 1F4‑1B5) LS‑C197963
Species: Human
Applications: Western blot, ELISA
PPIA / Cyclophilin A Antibody - PPIA / Cyclophilin A antibody PPIA(peptidylprolyl isomerase A (cyclophilin A)) Antibody was used in IHC to stain formalin-fixed, paraffin-embedded human intestine. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPIA / Cyclophilin A Antibody (C‑Terminus) LS‑C81638
Species: Human
Applications: IHC, IHC - Paraffin, Western blot
Product
PPIA / Cyclophilin A Antibody (Internal) LS‑C153408
Species: Human
Applications: IHC, Western blot, ELISA
PPIA / Cyclophilin A Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
PPIA / Cyclophilin A Antibody (Biotin) LS‑C212163
Species: Human, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PPIA / Cyclophilin A Antibody - Human Colon: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
IHC‑plus™ PPIA / Cyclophilin A Antibody LS‑B12256
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot, ELISA
PPIA / Cyclophilin A Antibody - Human Placenta: Formalin-Fixed, Paraffin-Embedded (FFPE)
IHC‑plus™ PPIA / Cyclophilin A Antibody LS‑B13852
Species: Human, Mouse, Rat
Applications: IHC, IHC - Paraffin, Western blot
See all PPIA / Cyclophilin A Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/21/2024
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