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Catalog Number Size Price
LS-C756594-10 10 µg $318 
LS-C756594-100 100 µg $470 
POU4F1 / BRN3A Antibody - Western blot analysis of BRN3A using anti-BRN3A antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: human placenta tissue lysates,Lane 2: human Caco-2 whole cell lysate,Lane 3: human A549 whole cell lysate,Lane 4: human Hela whole cell lysate. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BRN3A antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BRN3A at approximately 45KD. The expected band size for BRN3A is at 43KD.

Polyclonal Rabbit anti‑Human POU4F1 / BRN3A Antibody (WB) LS‑C756594

Polyclonal Rabbit anti‑Human POU4F1 / BRN3A Antibody (WB) LS‑C756594

Antibody:
POU4F1 / BRN3A Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C756594-10
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
POU4F1 / BRN3A Rabbit anti-Human Polyclonal Antibody
Application:
WB, ELISA
Reactivity:
Human, Mouse, Rat
Format:
Unconjugated, Unmodified

Specifications

Description
BRN3A antibody LS-C756594 is an unconjugated rabbit polyclonal antibody to BRN3A (POU4F1) from human. It is reactive with human, mouse and rat. Validated for ELISA and WB.
Target
Human POU4F1 / BRN3A
Synonyms
POU4F1 | Brain-3A | BRN3A | Brn-3A | Oct-T1 | POU class 4 homeobox 1 | RDC1 | RDC-1
Host
Rabbit
Reactivity
Human, Mouse, Rat (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived human BRN3A recombinant protein (Position: R311-Y419).
Specificity
No cross reactivity with other proteins.
Applications
  • Western blot (0.1 - 0.5 µg/ml)
  • ELISA (0.1 - 0.5 µg/ml)
Usage
Applications should be user optimized.
Presentation
Lyophilized from 0.2mg Na2HPO4, 0.9mg NaCl, 0.05mg sodium azide, 4mg Trehalose
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
After reconstitution, may be stored at 4°C for 1 month. For long-term storage and to avoid freeze-thaw cycles, aliquot and store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POU4F1 / BRN3A
Q01851 NM_006237 NP_006228.3

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 12/21/2024