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Antibodies > PON1 / ESA > LS-C661989
 
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Catalog Number Size Price
LS-C661989-10 10 µg $318 
LS-C661989-100 100 µg $470 
PON1 / ESA Antibody - IHC analysis of PON1 using anti-PON1 antibody. PON1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PON1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PON1 / ESA Antibody - IHC analysis of PON1 using anti-PON1 antibody. PON1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PON1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PON1 / ESA Antibody - IHC analysis of PON1 using anti-PON1 antibody. PON1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PON1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PON1 / ESA Antibody - Western blot analysis of PON1 using anti-PON1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse Liver tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PON1 at approximately 43KD. The expected band size for PON1 is at 40KD.
PON1 / ESA Antibody - Western blot - Anti-PON1/Paraoxonase 1 Picoband Antibody
PON1 / ESA Antibody - IHC analysis of PON1 using anti-PON1 antibody. PON1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PON1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PON1 / ESA Antibody - IHC analysis of PON1 using anti-PON1 antibody. PON1 was detected in paraffin-embedded section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PON1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PON1 / ESA Antibody - IHC analysis of PON1 using anti-PON1 antibody. PON1 was detected in paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-PON1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
PON1 / ESA Antibody - Western blot analysis of PON1 using anti-PON1 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: rat liver tissue lysates,Lane 2: mouse Liver tissue lysates,Lane 3: mouse lung tissue lysates,Lane 4: mouse testis tissue lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PON1 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PON1 at approximately 43KD. The expected band size for PON1 is at 40KD.
PON1 / ESA Antibody - Western blot - Anti-PON1/Paraoxonase 1 Picoband Antibody
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Polyclonal Rabbit anti‑Mouse PON1 / ESA Antibody (IHC, WB) LS‑C661989

Polyclonal Rabbit anti‑Mouse PON1 / ESA Antibody (IHC, WB) LS‑C661989

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Antibody:
PON1 / ESA Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C661989-10
Toll Free North America
206-374-1102
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
PON1 / ESA Rabbit anti-Mouse Polyclonal Antibody
Application:
IHC, WB
Reactivity:
Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
ESA antibody LS-C661989 is an unconjugated rabbit polyclonal antibody to mouse ESA (PON1). Validated for IHC and WB.
Target
Mouse PON1 / ESA
Synonyms
PON1 | A-esterase 1 | Aromatic esterase 1 | Esterase A | ESA | K-45 | MVCD5 | Paraoxonase 1 | PON 1 | Serum aryldialkylphosphatase 1 | Arylesterase B-type | Paraoxonase B-type | PON | Serum aryldiakylphosphatase
Host
Rabbit
Reactivity
Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
E. coli-derived mouse PON1 recombinant protein (Position: A30-D274).
Specificity
Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.
Applications
  • IHC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PON1 / ESA
P27169 NM_000446 NP_000437.3

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PON1 / ESA Antibody - Anti-PON1 / ESA antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 1:100.
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Applications: IHC, IHC - Paraffin, Western blot, Flow Cytometry
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Species: Human
Applications: Western blot
PON1 / ESA Antibody - Human Testis: Formalin-Fixed, Paraffin-Embedded (FFPE). This image was taken for the unconjugated form of this product. Other forms have not been tested.
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PON1 / ESA Antibody - PON1 antibody ARP41401_P050-NP_000437-PON1(paraoxonase 1) Antibody Western blot of fetal liver lysate. This image was taken for the unconjugated form of this product. Other forms have not been tested.
PON1 / ESA Antibody (C‑Terminus) LS‑C40324
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Applications: Western blot
See all PON1 / ESA Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 7/17/2024
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