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Antibodies > POLR3E / SIN > LS-C368900
 
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Catalog Number Size Price
LS-C368900-30 30 µl (1 mg/ml) $255 
LS-C368900-100 100 µl (1 mg/ml) $299 
LS-C368900-200 200 µl (1 mg/ml) $351 
POLR3E / SIN Antibody - Immunohistochemical analysis of POLR3E staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
POLR3E / SIN Antibody - Immunofluorescent analysis of POLR3E staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
POLR3E / SIN Antibody - Western blot analysis of POLR3E expression in HeLa (A); Jurkat (B); Ramos (C) whole cell lysates.
POLR3E / SIN Antibody - Immunohistochemical analysis of POLR3E staining in human prostate cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected with HRP and DAB as chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
POLR3E / SIN Antibody - Immunofluorescent analysis of POLR3E staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
POLR3E / SIN Antibody - Western blot analysis of POLR3E expression in HeLa (A); Jurkat (B); Ramos (C) whole cell lysates.
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Polyclonal Rabbit anti‑Human POLR3E / SIN Antibody (Internal, IHC, IF, WB) LS‑C368900

Polyclonal Rabbit anti‑Human POLR3E / SIN Antibody (Internal, IHC, IF, WB) LS‑C368900

Submit Review LSBio Guarantee
Antibody:
POLR3E / SIN Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified
Price
Catalog Number
$255
LS-C368900-30
Toll Free North America
(800) 227-6666
For Research Use Only
Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:
POLR3E / SIN Rabbit anti-Human Polyclonal (Internal) Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Monkey
Format:
Unconjugated, Unmodified

Specifications

Description
SIN antibody LS-C368900 is an unconjugated rabbit polyclonal antibody to SIN (POLR3E) (Internal) from human. It is reactive with human and monkey. Validated for ICC, IF, IHC and WB.
Target
Human POLR3E / SIN
Synonyms
POLR3E | KIAA1452 | RNA polymerase III subunit C5 | RPC5 | SIN
Host
Rabbit
Reactivity
Human, Monkey (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide encompassing a sequence within the central region of human POLR3E.
Epitope
Internal
Specificity
Recognizes endogenous levels of POLR3E protein.
Applications
  • IHC
  • IHC - Paraffin (1:100 - 1:200)
  • ICC (1:100 - 1:500)
  • Immunofluorescence
  • Western blot (1:500 - 1:1000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR3E / SIN
Q9NVU0 NM_018119 NP_060589.1

Publications (0)

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Featured Products

POLR3E / SIN Antibody - Western blot of POLR3E antibody
POLR3E / SIN Antibody (160‑240 aa, Internal) LS‑C385585
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Western blot, ELISA
POLR3E / SIN Antibody - Human Kidney: Formalin-Fixed, Paraffin-Embedded (FFPE)
POLR3E / SIN Antibody (aa191‑240) LS‑C119920
Species: Human, Mouse
Applications: IHC, Immunofluorescence, Peptide Enzyme-Linked Immunosorbent Assay
POLR3E / SIN Antibody - Immunofluorescence of paraformaldehyde-fixed A431, using POLR3E antibody at 1:500 dilution.
POLR3E / SIN Antibody LS‑C155686
Species: Human
Applications: ICC, Immunofluorescence, Western blot
POLR3E / SIN Antibody - Immunohistochemistry of paraffin-embedded human skin tissue using antibody at dilution of 1:100.
POLR3E / SIN Antibody LS‑C379502
Species: Human
Applications: IHC, IHC - Paraffin, ELISA
POLR3E / SIN Protein - 12.5% SDS-PAGE of human POLR3E stained with Coomassie Blue
Human POLR3E / SIN Protein (Recombinant GST) (Full Length) - LS-G34402
Source: Wheat
Tag: GST
POLR3E / SIN Protein - Purified recombinant protein POLR3E was analyzed by SDS-PAGE gel and Coomassie Blue Staining
Human POLR3E / SIN Protein (Recombinant Myc-DDK (Flag)) (Full Length) - LS-G71862
Source: Human
Tag: Myc-DDK (Flag)
See all POLR3E / SIN Antibodies

Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com
Requested From: United States
Date Requested: 1/10/2025
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United States

Tel: (800) 227-6666 / (650) 697-3600

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For technical assistance:
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