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Catalog Number Size Price
LS-C809956-100 100 µl (1 mg/ml) $379 
LS-C809956-200 200 µl (1 mg/ml) $421 
POLR1E Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
POLR1E Antibody - Western blot analysis of RPA49 using K562 whole cells lysates
POLR1E Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.
POLR1E Antibody - Western blot analysis of RPA49 using K562 whole cells lysates
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Polyclonal Rabbit anti‑Human POLR1E Antibody (IF, WB) LS‑C809956

Polyclonal Rabbit anti‑Human POLR1E Antibody (IF, WB) LS‑C809956

Antibody:
POLR1E Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified
Price
Catalog Number
$379
LS-C809956-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
POLR1E Rabbit anti-Human Polyclonal Antibody
Application:
IF, WB, Peptide-ELISA
Reactivity:
Human
Format:
Unconjugated, Unmodified

Specifications

Description
POLR1E antibody LS-C809956 is an unconjugated rabbit polyclonal antibody to human POLR1E. Validated for IF, Peptide-ELISA and WB.
Target
Human POLR1E
Synonyms
POLR1E | PRAF1 | RNA polymerase I subunit A49 | PAF53 | RP11-405L18.3
Host
Rabbit
Reactivity
Human (tested or 100% immunogen sequence identity)
Clonality
IgG Polyclonal
Conjugations
Unconjugated
Purification
Peptide affinity chromatography.
Modifications
Unmodified
Immunogen
A synthesized peptide derived from human RPA49.
Specificity
RPA49 Antibody detects endogenous levels of total RPA49.
Applications
  • Immunofluorescence (1:100 - 1:500)
  • Western blot (1:1000 - 1:3000)
  • Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)
Usage
For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.
Presentation
PBS, pH 7.4, 0.02% Sodium Azide, 50% Glycerol
Storage
Upon receipt, store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About POLR1E
Q9GZS1 NM_022490 NP_071935.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/25/2024