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Catalog Number Size Price
LS-C59521-100 100 µg (1 mg/ml) $574 
PMS2 Antibody - Anti-PMS2 Antibody - Western Blot. Western blot analysis is shown using Protein A Purified Mouse Monoclonal Anti-PMS2 antibody to detect human PMS2 protein present in H157 cell lysates. Approximately 5,10 and 30 ug of cell lysate was loaded on a 4-12% NuPage SDS-PAGE gel using MES buffer. The blot was incubated with a 1:1000 dilution of the antibody at room temperature followed by washing. A 1:20000 dilution of HRP conjugated Gt-anti-Mouse IgG preceded color development using Pierce Chemicals SuperSignal substrate. Comparison to a molecular weight marker (not shown) indicates a single band of ~96.0 kD corresponding to the expected molecular weight for human PMS2 protein. Other detection systems will yield similar results. Personal communication Morphotek Inc.

Monoclonal Mouse anti‑Human PMS2 Antibody (clone 1204, aa1‑133, WB) LS‑C59521

Monoclonal Mouse anti‑Human PMS2 Antibody (clone 1204, aa1‑133, WB) LS‑C59521

Antibody:
PMS2 Mouse anti-Human Monoclonal (aa1-133) (1204) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified
Price
Catalog Number
$574
LS-C59521-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PMS2 Mouse anti-Human Monoclonal (aa1-133) (1204) Antibody
Application:
WB, ELISA
Reactivity:
Human, Chimpanzee, Mouse, Rat, Hamster
Format:
Unconjugated, Unmodified

Specifications

Description
PMS2 antibody LS-C59521 is an unconjugated mouse monoclonal antibody to PMS2 (aa1-133) from human. It is reactive with human, mouse, rat and other species. Validated for ELISA and WB.
Target
Human PMS2
Synonyms
PMS2 | HNPCC4 | H_DJ0042M02.9 | PMS2CL | PMSL2 | PMS1 protein homolog 2
Host
Mouse
Reactivity
Human, Chimpanzee, Mouse, Rat, Hamster (tested or 100% immunogen sequence identity)
Clonality
IgG1,k Monoclonal
Clone
1204
Conjugations
Unconjugated
Purification
Protein A purified
Modifications
Unmodified
Immunogen
This protein A purified monoclonal antibody was produced by repeated immunizations with recombinant human PMS2 corresponding to the first 133 amino acid residues of the protein. The clone was produced using conventional hybridoma technology.
Epitope
aa1-133
Specificity
Reacts with full length version of PMS2 in human and hamster tissues. The epitope was putatively mapped to amino acids 58-81 of human PMS2. BLAST analysis indicates that this sequence is 100% identical for human, mouse, rat and chimpanzee. No specific information is available for reactivity with PMS2 protein from other sources.
Applications
  • Western blot (1:500 - 1:2000)
  • ELISA (1:5000 - 1:20000)
Usage
This antibody has been tested for use in ELISA, western blotting and immunoprecipitation. Specific conditions for western blotting reactivity should be optimized by the end user. Expect a band approximately 96 kD in size corresponding to human PMS2 by western blotting in most cell lines and tissues as PMS2 is ubiquitously expressed.
Presentation
0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PMS2
P54278 NM_000535 NP_000526.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 11/21/2024