Target
Human PLXND1 / Plexin D1
Synonyms
PLXND1 | KIAA0620 | PLEXD1 | Plexin D1 | Plexin-D1
Reactivity
Human
(tested or 100% immunogen sequence identity)
Purification
Immunoaffinity purified
Immunogen
NS0-derived recombinant human rhPlexin D1 extracellular domain.
Epitope
Extracellular Domain
Specificity
Recognizes human Plexin D1 extracellular domain. Crossreactivity: rmPlexin A1, rmPlexin A3, and rhPlexin B1 <1%.
Applications
- Western blot
- Flow Cytometry (25 µg/ml)
Usage
Suitable for use in Western Blot and Flow cytometry. Western blot: 0.1 0.2 ug/ml. The detection limit is ~1 ng/lane under non-reducing and reducing conditions. Flow cytometry: 25 ug/ml. Add 10il of the diluted solution to 1-2.5 x 10^5 cells in a total reaction volume not exceeding 200ul. The binding of biotinylated antibodies may be visualized by adding a secondary developing reagent such as streptavidin conjugated to a fluorochrome. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from PBS, BSA
Reconstitution
TBS, pH7.3, containing 0.1% BSA
Storage
Lyophilized powder may be stored at 4°C for short-term only. Reconstituted product is stable for 1 year at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee