Target
Human PLN / Phospholamban
Synonyms
PLN | CMD1P | CMH18 | PLB | Cardiac phospholamban | Phospholamban
Reactivity
Human, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Immunogen
Synthetic peptide corresponding to aa14-25 of human cardiac phospholamban with a cysteine added at the C-Terminus (RA[pS]TIEMPQQAR-C) (KLH).
Specificity
Recognizes human Phospholamban phosphorylated (Ser16) (Mr 5-25kD). Species cross-reactivity: bovine. Species sequence Homology: mouse, rat, rabbit, porcine and canine.
Applications
- Western blot (0.5 - 2 µg/ml)
- ELISA
Usage
Suitable for use in ELISA and Western Blot. Proteins were visualized using a goat anti-rabbit IgG labeled with HRP and a chemiluminescence detection system. Note: Do not boil cardiac preparation. Boiling may reduce the 25kD pentamer in to tri-, di- or monomers. In addition, phosphorylation may disrupt the pentamer into dimers and monomers. Additional non-specific bands may be detected in some lysates (Mr 75 & 100kD).
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PLN / Phospholamban