Target
Human PLIN3 / M6PRBP1 / TIP47
Synonyms
PLIN3 | 47 kDa MPR-binding protein | Cargo selection protein TIP47 | Perilipin 3 | Placental protein 17 | PP17 | M6PRBP1 | TIP47 | Perilipin-3
Reactivity
Human, Monkey
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence C-TRGAVQSGVDKTKS, from the internal region of the protein sequence according to NP_005808.3NP_001157661.1NP_001157666.1.
Specificity
Human PLIN3 / M6PRBP1 / TIP47. This antibody is expected to recognize all reported isoforms (NP_005808.3; NP_001157661.1; NP_001157666.1).
Applications
- Western blot (0.03 - 0.1 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:128000)
Usage
Peptide ELISA: antibody detection limit dilution 1:128000. Western blot: Approx 48kD+45kD double bands observed in lysates of cell line HeLa and of Human Colon cancer (calculated MW of 47.1kD according to NP_NP_005808.3, 46.9kD according to NP_001157661.1 and 45.8kD according to NP_001157666.1). The observed bands represent the three different isoforms. Please note the difference in band pattern intensities between HeLa and Colon cancer lysates. Recommended concentration: 0.03-0.1 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PLIN3 / M6PRBP1 / TIP47