Reactivity
Human
(tested or 100% immunogen sequence identity)
Conjugations
FITC.
Also available
Unconjugated or conjugated with
TRITC.
Purification
Purified IgG
Immunogen
Purified normal IgM isolated from pooled human serum. Freund's complete adjuvant is used in the first step of the immunization procedure.
Specificity
Human IgM, Fc specific.
Applications
- IHC
- IHC - Frozen
- ICC
- Immunofluorescence
- ELISA
- Immunodiffusion
- Applications tested for the base form of this product only
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested. The reactivity of the antiserum is directed to the Fc subunits of the IgM molecule which expresses strict isotypic specificity. The conjugate does not react with any non-Ig protein in human serum, as tested by immunoelectrophoresis and double radial immunodiffusion. To identify IgM at the cellular and subcellular level by immunofluorescence staining of appropriately treated cell and tissue substrates, and to demonstrate circulating antibodies in serodiagnostic microbiology and autoimmune diseases. This immunoconjugate is not pre-diluted. The optimum working dilution of each conjugate should be established by titration before being used. Excess labeled antibody must be avoided because it may cause high unspecific background staining and interfere with the specific signal. Working dilutions are usually between 1:20 and 1:80.
Presentation
Lyophilized from PBS, pH 7.2, No preservatives added
Reconstitution
Reconstitute in 1ml sterile distilled water.
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee