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Catalog Number Size Price
LS-C57996-0.5 0.5 ml $561 
Antibody

Monoclonal Mouse anti‑Synthetic Phosphotyrosine Antibody (clone PY20, HRP) LS‑C57996

Monoclonal Mouse anti‑Synthetic Phosphotyrosine Antibody (clone PY20, HRP) LS‑C57996

Antibody:
Phosphotyrosine Mouse anti-Synthetic Monoclonal (HRP) (PY20) Antibody
Application:
ELISA
Reactivity:
Synthetic
Format:
Horseradish Peroxidase, Unmodified
Price
Catalog Number
$561
LS-C57996-0.5
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
Phosphotyrosine Mouse anti-Synthetic Monoclonal (HRP) (PY20) Antibody
Application:
ELISA
Reactivity:
Synthetic
Format:
Horseradish Peroxidase, Unmodified

Specifications

Description
Phosphotyrosine antibody LS-C57996 is an HRP-conjugated mouse monoclonal antibody to synthetic Phosphotyrosine. Validated for ELISA.
Target
Synthetic Phosphotyrosine
Host
Mouse
Reactivity
Synthetic (tested or 100% immunogen sequence identity)
Clonality
IgG2b Monoclonal
Clone
PY20
Conjugations
Horseradish Peroxidase
Purification
Affinity purified
Modifications
Unmodified
Specificity
Recognizes phosphotyrosine, enabling the detection, characterization and isolation of proteins containing phosphorylated tyrosine residues. The phosphorylation of tyrosine acts as a important signal in the control of cell mitogenesis, differentiation, proliferation, and migration and occurs following the activation of intracellular tyrosine kinases, including the T-cell receptor (TCR), epidermal growth factor (EGF) and many families of receptor and non-receptor protein tyrosine kinases (PTKs), which catalyze the transfer of ATP to a tyrosine residue on specific cell protein targets. The binding of PY20 to phosphorylated tyrosines can be inhibited by free phosphotyrosine and phenylphosphate, but not by free phosphate, phosphoserine or phosphothreonine. The affinity of PY20 for phosphotyrosine is 10-6 to 10-7 M.
Applications
  • ELISA (1:1000 - 1:4000)
Usage
The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
PBS, 0.01% Thimerosal, 50% Glycerol
Storage
Store at 4°C or at -20°C. Store undiluted. Avoid freeze-thaw cycles. Microcentrifugation recommended if solution contains precipitate.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 3/13/2025