Phosphotyrosine

Mouse

IgG2b Monoclonal

PY20

Unconjugated

Protein G purified

Azide-free, BSA-free

Phosphotyrosine conjugated to KLH.

Phosphotyrosine

• IHC
• IHC - Paraffin (1 - 2 µg/ml)
• Immunofluorescence (1 - 2 µg/ml)
• Western blot (0.5 - 1 µg/ml)
• Immunoprecipitation
• Flow Cytometry (0.5 - 1 µg/10E6 cells)

Protein phosphorylation is a fundamental event in the regulation of a large number of intracellular processes. Phosphorylation of specific tyrosine residues is the result of activation or stimulation of their respective protein tyrosine kinases. The phosphorylated proteins can be auto-phosphorylated kinases or certain cellular protein substrates. Tyrosine-phosphorylated proteins are involved in signal transduction and in the regulation of cell proliferation. antibody to phosphotyrosine provides an excellent tool for the detection, characterization, and purification of phosphotyrosine containing proteins. This antibody shows no cross-reaction with other phosphoamino acids and is superb for multiple applications including staining of formalin/paraffin tissues. ELISA: Order BSA-free format for coating. The applications listed have been tested for the unmodified form of this product. Other forms have not been tested.

1X PBS

Store at 2°C to 8°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.