Target
Rabbit PFKM / PFK-1
Synonyms
PFKM | 6-phosphofructo-1-kinase | GSD7 | PFK1 | PFKA | PFKX | Phosphofructokinase-M | Phosphofructokinase 1 | PFK-1 | PFK-A | Phosphofructokinase, muscle | Phosphohexokinase
Reactivity
Rabbit
(tested or 100% immunogen sequence identity)
Conjugations
Horseradish Peroxidase
Purification
Delipidation, salt fractionation and ion exchange chromatography followed by dialysis.
Modifications
Lyophilized
Immunogen
Fructose-6-Phosphate Kinase [Rabbit Muscle].
Specificity
Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-Peroxidase, anti-Goat Serum as well as purified and partially purified Fructose-6-Phosphate Kinase [Rabbit Muscle]. Cross reactivity against Fructose-6-Phosphate Kinase from other sources may occur but have not been specifically determined.
Applications
- Western blot (1:500 - 1:2000)
- ELISA (1:4000 - 1:20000)
Usage
Anti-Fructose-6-Phosphate Kinase antibody has been tested for use in ELISA, immunofluorescence microscopy and western blot. Expect a band approximately 48 kD in size corresponding to the processed mature form of F6PK protein by western blotting in the appropriate cell lysate or extract. The applications listed have been tested for the unconjugated form of this product. Other forms have not been tested.
Presentation
Lyophilized from 10 mg/mL BSA (Protease and Ig free), 0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Gentamicin. Do NOT add Sodium Azide
Reconstitution
Reconstitute in 100 µL of deionized water.
Storage
Short term 4°C, long term aliquot and store at -20°C, avoid freeze-thaw cycles. Centrifuge product before removing cap. Only dilute immediately prior to use.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee