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| Catalog Number | Size | Price |
|---|---|---|
| LS-B1527-50 | 50 µl (0.5 mg/ml) | $460 |
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Polyclonal Rabbit anti‑Human PEA3 / ETV4 Antibody (aa181‑230, IHC, WB) LS‑B1527
Polyclonal Rabbit anti‑Human PEA3 / ETV4 Antibody (aa181‑230, IHC, WB) LS‑B1527
Note: This antibody replaces LS-C29900
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Specifications
Description
ETV4 antibody LS-B1527 is an unconjugated rabbit polyclonal antibody to human ETV4 (PEA3) (aa181-230). Validated for IHC and WB. Cited in 2 publications.
Target
Human PEA3 / ETV4
Synonyms
ETV4 | ETS translocation variant 4 | E1A-F | E1AF | Ets variant 4 | PEA3 | Protein PEA3 | PEAS3
Host
Rabbit
Reactivity
Human
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
Synthetic peptide located between aa181-230 of human ETV4 (P43268, NP_001977). Percent identity by BLAST analysis: Human, Chimpanzee, Gorilla, Monkey, Galago, Mouse, Rat, Elephant, Bovine, Pig (100%); Marmoset, Bat, Rabbit, Horse (92%).
Epitope
aa181-230
Specificity
Human ETV4 / PEA3
Applications
- IHC
- IHC - Paraffin (5 µg/ml)
- Western blot (0.2 - 1 µg/ml)
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
Immunohistochemistry: LS-B1527 was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary antibody, slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase-streptavidin and chromogen. The stained slides were evaluated by a pathologist to confirm staining specificity. The optimal working concentration for LS-B1527 was determined to be 5 ug/ml.
Presentation
PBS, 0.09% sodium azide, 2% sucrose
Storage
Short term: Store at 2-8°C. Long term: Aliquot and store at -20°C. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PEA3 / ETV4
LSBio Ratings
PEA3 / ETV4 Antibody (aa181-230) for IHC, WB/Western LS-B1527 has an LSBio Rating of
Publications (4.1)
Learn more about The LSBio Ratings Algorithm
Publications (2)
Inactivation of Capicua drives cancer metastasis. Okimoto RA, Breitenbuecher F, Olivas VR, Wu W, Gini B, Hofree M, Asthana S, Hrustanovic G, Flanagan J, Tulpule A, Blakely CM, Haringsma HJ, Simmons AD, Gowen K, Suh J, Miller VA, Ali S, Schuler M, Bivona TG. Nature genetics. 2017 49:87-96. (WB; Mouse, Human)
ME1 Regulates NADPH Homeostasis to Promote Gastric Cancer Growth and Metastasis. Lu YX, Ju HQ, Liu ZX, Chen DL, Wang Y, Zhao Q, Wu QN, Zeng ZL, Qiu HB, Hu PS, Wang ZQ, Zhang DS, Wang F, Xu RH. Cancer research. 2018 April;78:1972-1985.
Customer Reviews (0)
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Species:
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Species:
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IHC, IHC - Paraffin, Peptide Enzyme-Linked Immunosorbent Assay
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
Date Requested: 12/26/2024
