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Catalog Number Size Price
LS-C482390-50 50 µl (1 mg/ml) $286 
LS-C482390-100 100 µl (1 mg/ml) $321 
LS-C482390-200 200 µl (1 mg/ml) $386 
PDK4 Antibody - Immunohistochemical analysis of PDK4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PDK4 Antibody - Immunofluorescent analysis of PDK4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PDK4 Antibody - Western blot analysis of PDK4 expression in THP1 (A); HeLa (B); mouse kidney (C); mouse heart (D) whole cell lysates.
PDK4 Antibody - Immunohistochemical analysis of PDK4 staining in human colon cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with hematoxylin and mounted with DPX.
PDK4 Antibody - Immunofluorescent analysis of PDK4 staining in U2OS cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 deg C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
PDK4 Antibody - Western blot analysis of PDK4 expression in THP1 (A); HeLa (B); mouse kidney (C); mouse heart (D) whole cell lysates.
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Polyclonal Rabbit anti‑Human PDK4 Antibody (IHC, IF, WB) LS‑C482390

Polyclonal Rabbit anti‑Human PDK4 Antibody (IHC, IF, WB) LS‑C482390

Antibody:
PDK4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$286
LS-C482390-50
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
PDK4 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, ICC, IF, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
PDK4 antibody LS-C482390 is an unconjugated rabbit polyclonal antibody to PDK4 from human. It is reactive with human and mouse. Validated for ICC, IF, IHC and WB.
Target
Human PDK4
Synonyms
PDK4 | PDHK4
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunoaffinity purified
Modifications
Unmodified
Immunogen
KLH-conjugated synthetic peptide of human PDK4.
Specificity
Recognizes endogenous levels of PDK4 protein.
Applications
  • IHC
  • IHC - Paraffin (1:50 - 1:200)
  • ICC (1:10 - 1:100)
  • Immunofluorescence (1:10 - 1:100)
  • Western blot (1:500 - 1:2000)
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
PBS, pH 7.3, 0.01% Sodium Azide, 30% Glycerol
Storage
Aliquot and store at -20°C for 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About PDK4
Q16654 NM_002612 NP_002603.1

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To request an SDS/MSDS form for this product, please contact our Technical Support department at:

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Requested From: United States
Date Requested: 11/23/2024