Human PDGFRA / PDGFR Alpha

Mouse

Human (tested or 100% immunogen sequence identity)

IgG1 Monoclonal

35248 (57/20)

Unconjugated

Protein G purified

Unmodified

Purified recombinant human platelet-derived growth factor (PDGF) receptor alpha (extracellular domain), derived from the insect cell line Sf21.

Extracellular Domain

Recognizes the human platelet-derived growth factor receptor alpha, a 1089 amino acid, ~180 kD single pass type I transmembrane glycoprotein. PDGF is a major serum mitogen that can exist as a homo- or heterodimeric protein consisting of disulphide-linked PDGF-A and PDGF-B chains. The PDGF-AA, PDGF-BB and PDGF-AB isoforms have been shown to bind to two distinct cell surface PDGF receptors with different affinities. Whereas PDGF receptor alpha binds all three isoforms with high affinity, PDGF R beta binds PDGF-BB and AB but not PDGF-AA. Both PDGF receptor alpha and beta are members of the class III subfamily of receptor tyrosine kinases. Has been reported for use in functional studies. The ND50 was determined as 0.2-0.6 Âug/ml for blocking 10 ng/ml of PDGF-AA biological activity on a quiescent confluent cell culture of human WS1 cells.

• IHC
• IHC - Paraffin (10 - 20 µg/ml)
• Immunofluorescence
• Western blot (1 - 2 µg/ml)
• ELISA (0.5 - 1 µg/ml)

PBS, 0.09% Sodium Azide, 5% Trehalose

Store at 4°C or at -20°C. Store undiluted. Avoid freeze/thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P16234 NCBI: NM_006206 NP_006197.1