Mouse PDCD1 / CD279 / PD-1

Goat

Mouse (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Immunoaffinity purified

Unmodified

Sf21-derived rmPD-1 extracellular domain.

Extracellular Domain

Recognizes mouse PD-1 extracellular domain. Shows approximately 50% cross-reactivity with rhPD-1.

• IHC
• Western blot
• Flow Cytometry
• ELISA

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA. Western Blot: This antibody can be used at 0.1-0.2 ug/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 5 ng/lane under non-reducing and reducing conditions. Immunohistochemistry: This antibody will detect PD-1 in cells and tissues. The working dilution is 2-15 ug/mL. Flow Cytometry: This antibody can be used at 3-10 ug/mL/1E6 cells with an appropriate secondary antibody for indirect immunofluorescence staining of cells by flow cytometry. Direct ELISA: This antibody can be used at 0.5-1.0 ug/mL with the appropriate secondary reagents to detect mouse PD-1. The detection limit for rmPD-1 is approximately 0.03 ng/well.

Lyophilized from PBS, pH 7.4, 5% Trehalose

0.5 ml sterile PBS

Store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: Q15116 NCBI: NM_005018 NP_005009.2