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| Catalog Number | Size | Price |
|---|---|---|
| LS-B540-0.05 | 0.05 mg (1 mg/ml) | $460 |
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IHC‑plus™ Polyclonal Rabbit anti‑Human PDCD1 / CD279 / PD‑1 Antibody (IHC, IF, WB) LS‑B540
IHC‑plus™ Polyclonal Rabbit anti‑Human PDCD1 / CD279 / PD‑1 Antibody (IHC, IF, WB) LS‑B540
Note: This antibody replaces LS-C19688
Antibody:
PDCD1 / CD279 / PD-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Toll Free North America
(800) 227-6666
(800) 227-6666For Research Use Only
Overview
Antibody:
PDCD1 / CD279 / PD-1 Rabbit anti-Human Polyclonal Antibody
Application:
IHC, IHC-P, IF, WB, ELISA
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Specifications
Description
PD-1 antibody LS-B540 is an unconjugated rabbit polyclonal antibody to PD-1 (PDCD1 / CD279) from human. It is reactive with human and mouse. Validated for ELISA, IF, IHC and WB. Tested on 20 paraffin-embedded human tissues. Cited in 1 publication.
Target
Human PDCD1 / CD279 / PD-1
Synonyms
PDCD1 | CD279 | HPD-1 | HPD-l | PD1 | Protein PD-1 | SLEB2 | PD-1 | CD279 antigen | Programmed cell death 1
Host
Rabbit
Reactivity
Human, Mouse
(tested or 100% immunogen sequence identity)
Clonality
IgG
Polyclonal
Conjugations
Unconjugated
Purification
Peptide Column Affinity Chromatography
Modifications
Unmodified
Immunogen
A 16 AA synthetic peptide near the C-Terminus of human PD-1. The immunogen is located within aa 210-260 of PD-1.
Applications
- IHC
- IHC - Paraffin (2.5 µg/ml)
- Immunofluorescence
- Western blot
- ELISA
|
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents
ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
|
Usage
PD-1 antibody can be used for detection of PD-1 by Western blot at 1 ug/mL. Antibody can also be used for immunohistochemistry starting at 2.5 ug/mL. For immunofluorescence start at 20 ug/mL. Antibody validated: Western Blot in human samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested. Western Blot: Predicted: 32 kDa Observed: 45 kDa
Presentation
PBS containing 0.02% Sodium Azide
Storage
Store at 4°C for up 3 months. Store at -20°C for up to 1 year. Avoid freeze/thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
About PDCD1 / CD279 / PD-1
Validation
Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
Anti-PD1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.
See More About...
LSBio Ratings
IHC-plus™ PDCD1 / CD279 / PD-1 Antibody for IHC, IF/Immunofluorescence, WB/Western, ELISA LS-B540 has an LSBio Rating of
Publications (4)
Laboratory Validation Score (4)
Learn more about The LSBio Ratings Algorithm
Publications (1)
Somatic Mutations and Immune Alternation in Rectal Cancer Following Neoadjuvant Chemoradiotherapy. Dengbo Ji , Haizhao Yi , Dakui Zhang , Tiancheng Zhan, Zhaowei Li, Ming Li, Jinying Jia, Meng Qiao, Jinhong Xia, Zhiwei Zhai, Can Song, Jin Gu. Cancer immunology research. 2018 Nov;6:1401-1416.
Customer Reviews (0)
Featured Products
Species:
Human
Applications:
IHC, IHC - Paraffin, IHC - Frozen, Western blot, Immunoprecipitation, Flow Cytometry
Species:
Human, Mouse, Rat
Applications:
IHC, IHC - Paraffin, ICC, Immunofluorescence, Western blot, ELISA
Species:
Human
Applications:
Flow Cytometry
Species:
Human
Applications:
IHC, IHC - Paraffin, Flow Cytometry
Species:
Human
Applications:
Western blot, ELISA
Reactivity:
Human
Range:
0.156-10 ng/ml
Request SDS/MSDS
To request an SDS/MSDS form for this product, please contact our Technical Support department at:
Technical.Support@LSBio.com
Requested From: United States
Date Requested: 12/26/2024
Date Requested: 12/26/2024
