Target
Human PCSK9
Synonyms
PCSK9 | FH3 | HCHOLA3 | LDLCQ1 | NARC-1 | Proprotein convertase 9 | NARC1 | PC9
Reactivity
Human, Monkey, Mouse, Rat, Hamster
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Purification
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Immunogen
Peptide with sequence TRFHRQASKCDSHGT, from the internal region of the protein sequence according to NP_777596.2.
Applications
- Western blot (0.1 - 0.5 µg/ml)
- Peptide Enzyme-Linked Immunosorbent Assay (1:16000)
Usage
Peptide ELISA: antibody detection limit dilution 1:16000. Western blot: In transfected HEK293 transiently expressing full-length Human PCSK9 (myc and DYKDDDDK tagged), a doublet of approx. 80-100kD was observed. No bands were observed in mock-transfected HEK293 and the same bands were observed using anti-myc tag antibody. Recommended concentration, 0.1-0.5 ug/ml.
Presentation
TBS, pH 7.3, 0.02% Sodium Azide, 0.5% BSA
Storage
Aliquot and store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee