Target
Human p56lck / LCK
Synonyms
LCK | Lck kinase | LSK | p56-LCK | Pp58lck | Tyrosine-protein kinase Lck | p56lck | Protein YT16 | Proto-oncogene Lck | YT16
Reactivity
Human, Mouse, Bovine
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated.
Also available conjugated with
APC,
Biotin,
FITC,
HRP,
PE.
Purification
Protein A purified
Immunogen
GST fusion protein corresponding to aa1-58 of human Lck.
Specificity
Recognizes Lck, Mr 56kD of mouse, human and bovine; reactivity with other Src family members not determined.
Applications
- Western blot
- Immunoprecipitation
Usage
Western Blot Analysis: 0.5-2 ug/ml detected Lck in RIPA lysates from Jurkat cells. Included Positive Antigen Control: Jurkat cell lysate. Use 20 ug per lane for minigels. Immunoprecipitation: 4 ug immunoprecipitated Lck from 1 ug of Jurkat RIPA lysate as confirmed by subsequent immunoblot using monoclonal anti-Lck. Western Blot Analysis Jurkat cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with anti-Lck (1 ug/ml). Proteins were visualized using a goat anti rabbit secondary antibody conjugated to HRP and a chemiluminescence detection system.
Presentation
0.1 M Tris-Glycine, pH 7.4, 0.15 M NaCl, 0.05% Sodium Azide
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee