Rat P2RX3 / P2X3

Rabbit

Rat, Mouse, Hamster (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Antiserum

Unmodified

Synthetic peptide corresponding to aa383-397 (VEKQSTDSGAYSIGH) of rat P2X3. Percent identity by BLAST analysis: Mouse, Rat, Hamster (100%); Elephant, Panda, Bovine, Dog, Horse (93%); Human, Gorilla, Gibbon, Monkey, Marmoset, Bat, Rabbit, Opossum (87%).

aa383-397

Recognizes rat P2X3 Receptor. Species cross-reactivity: Human and primate.

• IHC
• ICC (1:500)
• Immunofluorescence
• Western blot (1:500)

Suitable for use in Western Blot, Immunohistochemistry and Immunocytochemistry. Membranes were rinsed with PBS and incubated with Goat anti-rat IgG labeled with HRP for 1 hour at RT. Blocking buffer was removed. Slides were incubated for 18-24 hours at 4?C with primary antiserum. Slides were rinsed 3 times with PBS. Slides were incubated with secondary antibodies for 1 hour at RT. Slides were again rinsed 3 times with PBS and coverslipped. Staining was examined using Fluorescence Microscopy. Immunocytochemistry: 1:500. P2X3 transfected cells were processed for Indirect Immunofluorescence. Media was removed. Cells were gently washed 3 times with serum-free media. Following fixation procedure, cells were processed for Indirect Immunofluorescence as described for Immunohistochemistry.

PBS, 0.05% Sodium Azide

Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P56373 NCBI: NM_002559 NP_002550.2