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Catalog Number Size Price
LS-C820931-100 100 µg (1 mg/ml) $534 
O-GalNAC Antibody - Immunohistochemistry analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite.
O-GalNAC Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
O-GalNAC Antibody - Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
O-GalNAC Antibody - Immunohistochemistry analysis using Mouse Anti-O-GalNAc Monoclonal Antibody, Clone 9B9. Tissue: colon. Species: Mouse. Fixation: Formalin fixed, paraffin embedded. Primary Antibody: Mouse Anti-O-GalNAc Monoclonal Antibody at 1:25 for 1 hour at RT. Secondary Antibody: Goat Anti-Mouse IgG: Alexa Fluor 488. Counterstain: Actin-binding Phalloidin-Alexa Fluor 633; DAPI (blue) nuclear stain. Magnification: 63X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite.
O-GalNAC Antibody - Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Tissue: Embryonic kidney epithelial cell line (HEK293). Species: Human. Fixation: 5% Formaldehyde for 5 min. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:50 for 30-60 min at RT. Secondary Antibody: Goat Anti-Mouse Alexa Fluor 488 at 1:1500 for 30-60 min at RT. Counterstain: Phalloidin Alexa Fluor 633 F-Actin stain; DAPI (blue) nuclear stain at 1:250, 1:50000 for 30-60 min at RT. Magnification: 20X (2X Zoom). (A) DAPI (blue) nuclear stain. (B) Phalloidin Alexa Fluor 633 F-Actin stain. (C) O-GalNAc Antibody (D) Composite. Courtesy of: Dr. Robert Burke, University of Victoria.
O-GalNAC Antibody - Western Blot analysis of Glycoconjugates showing detection of 67 kDa O-GalNAC protein using Mouse Anti-O-GalNAC Monoclonal Antibody, Clone 9B9. Lane 1: Molecular Weight Ladder (MW). Lane 2: GlcNAc-BSA. Lane 3: GalNAc-BSA. Lane 4: Galactose-BSA. Lane 5: Glucose-BSA. Load: 2.0 µg. Block: 5% Skim Milk in TBST. Primary Antibody: Mouse Anti-O-GalNAC Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse IgG: HRP at 1:2000 for 60 min at RT. Color Development: ECL solution for 5 min in RT. Predicted/Observed Size: 67 kDa.
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Monoclonal Mouse anti‑All species O‑GalNAC Antibody (clone 9B9, Atto 594, IHC, IF, WB) LS‑C820931

Monoclonal Mouse anti‑All species O‑GalNAC Antibody (clone 9B9, Atto 594, IHC, IF, WB) LS‑C820931

Antibody:
O-GalNAC Mouse Monoclonal (Atto 594) (9B9) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
All species
Format:
Atto 594, Unmodified
Other formats:
Price
Catalog Number
$534
LS-C820931-100
Toll Free North America
(800) 227-6666
For Research Use Only

Overview

Antibody:
O-GalNAC Mouse Monoclonal (Atto 594) (9B9) Antibody
Application:
IHC, IF, WB, ELISA
Reactivity:
All species
Format:
Atto 594, Unmodified
Other formats:

Specifications

Description
O-GalNAC antibody LS-C820931 is an Atto 594-conjugated mouse monoclonal antibody to all species O-GalNAC. Validated for ELISA, IF, IHC and WB.
Target
All species O-GalNAC
Synonyms
O-GalNAC
Host
Mouse
Reactivity
All species
Clonality
IgM Monoclonal
Clone
9B9
Conjugations
Atto 594. Also available conjugated with Atto 390, Atto 488, APC, Biotin, FITC, PerCP, RPE.
Purification
PEG purified
Modifications
Unmodified
Immunogen
Synthetic ß-O-Linked N-Acetylglucosamine conjugated to Keyhole Limpet Hemocyanin (KLH).
Specificity
Specific for ß-O-Linked N-Acetylgalactosamine and ß-O-Linked N-Acetylglucosamine modified proteins. Does not cross-react with Glucose or Galactose modified proteins.
Applications
  • IHC (1:25)
  • Immunofluorescence (1:50)
  • Western blot (1:1000)
  • ELISA (1:1000)
  • Applications tested for the base form of this product only
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Usage
A 1:1000 dilution of the antibody was sufficient for detection of O-GlcNAc in 2 µg of O-GlcNAc conjugated to BSA by ECL immunoblot analysis using Goat Anti-Mouse IgG:HRP as the secondary Antibody. Applications should be user optimized.
Presentation
PBS, pH 7.4, 0.09% Sodium Azide, 50% Glycerol
Storage
Store at -20°C.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee

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Requested From: United States
Date Requested: 11/29/2024