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Catalog Number	Size	Price
LS-C801062-100	100 µl (1 mg/ml)	$379
LS-C801062-200	200 µl (1 mg/ml)	$421

NOS3 / eNOS Antibody - Phospho-eNOS (Ser1177) antibody antibody for IHC in human colon tissue

NOS3 / eNOS Antibody - 1:200 staining mouse intestinal tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22°C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.

NOS3 / eNOS Antibody - Staining K562 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 min at 25°C. The primary antibody was diluted at 1:200 and incubated with the sample for 1 hour at 37°C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.

NOS3 / eNOS Antibody - Staining HeLa cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 min at 37°C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37°C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.

NOS3 / eNOS Antibody - Western blot analysis of eNOS phosphorylation expression in Insulin treated HeLa whole cells lysates. The lane on the left is treated with the antigen-specific peptide.

NOS3 / eNOS Antibody - Western blot analysis of Phospho-eNOS (Ser1177) expression in various lysates

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Polyclonal Rabbit anti‑Human NOS3 / eNOS Antibody (phospho‑Ser1177, IHC, IF, WB) LS‑C801062

Name: Anti-NOS3 / eNOS Antibody | Rabbit anti-Human Polyclonal | LSBio
Brand: Lifespan BioSciences
SKU: LS-C801062-100
Price: 379.0000 USD
Availability: InStock

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Antibody:

NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

$379

LS-C801062-100

Toll Free North America

(800) 227-6666

For Research Use Only

Specifications Validation Publications Reviews Featured Products Request SDS/MSDS

Overview

Antibody:

NOS3 / eNOS Rabbit anti-Human Polyclonal (pSer1177) Antibody

Application:

IHC, IF, WB, Peptide-ELISA

Reactivity:

Human, Mouse, Rat

Format:

Unconjugated, Unmodified

Specifications

Description

ENOS antibody LS-C801062 is an unconjugated rabbit polyclonal antibody to eNOS (NOS3) (pSer1177) from human. It is reactive with human, mouse and rat. Validated for IF, IHC, Peptide-ELISA and WB.

Target

Human NOS3 / eNOS

Synonyms

See All NOS3 / eNOS Antibodies

Host

Rabbit

Reactivity

Human, Mouse, Rat (tested or 100% immunogen sequence identity)

Clonality

IgG Polyclonal

Conjugations

Unconjugated

Purification

Affinity purification via sequential chromatography on phospho- and non-phospho-peptide affinity columns.

Modifications

Unmodified

Immunogen

A synthesized peptide derived from human eNOS around the phosphorylation site of Serine 1177.

Epitope

pSer1177

Specificity

Phospho-eNOS (Ser1177) Antibody detects endogenous levels of eNOS only when phosphorylated at Serine 1177.

Applications

IHC (1:50 - 1:200)
Immunofluorescence
Western blot (1:500 - 1:2000)
Peptide Enzyme-Linked Immunosorbent Assay (1:20000 - 1:40000)

Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.

Usage

For western blots: incubate membrane with diluted antibody overnight in 5% w/v milk , 1X TBS, 0.1% Tween-20 at 4°C with gentle shaking.

Presentation

PBS, pH 7.4, 50% Glycerol

Storage

Upon receipt, store at -20°C. Avoid freeze-thaw cycles.

Restrictions

For research use only. Intended for use by laboratory professionals.

Guarantee

This antibody carries the LSBio 100% Guarantee.

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About NOS3 / eNOS