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Catalog Number Size Price
LS-C312974-10 10 µg $318 
LS-C312974-100 100 µg (0.5 mg/ml) $470 
NME1 / NM23 Antibody - IHC analysis of NM23A using anti-NM23A antibody. NM23A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NM23A Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NME1 / NM23 Antibody - NME1 / NM23 antibody. IHC(P): Rat Cerebellum Tissue.
NME1 / NM23 Antibody - NME1 / NM23 antibody. ICC: HELA Cell.
NME1 / NM23 Antibody - WB of NME1 / NM23 antibody. All lanes: Anti-NME1 at 0.5ug/ml. Lane 1: Rat Heart Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: Rat Liver Tissue Lysate at 40ug. Lane 4: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 5: PANC Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Lane 7: SKOV Whole Cell Lysate at 40ug. Lane 8: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 17KD. Observed bind size: 17KD.
NME1 / NM23 Antibody - Flow Cytometry analysis of Hela cells using anti-NME1 antibody. Overlay histogram showing Hela cells stained with anti-NME1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NME1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
NME1 / NM23 Antibody - IHC analysis of NM23A using anti-NM23A antibody. NM23A was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1µg/ml rabbit anti-NM23A Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
NME1 / NM23 Antibody - NME1 / NM23 antibody. IHC(P): Rat Cerebellum Tissue.
NME1 / NM23 Antibody - NME1 / NM23 antibody. ICC: HELA Cell.
NME1 / NM23 Antibody - WB of NME1 / NM23 antibody. All lanes: Anti-NME1 at 0.5ug/ml. Lane 1: Rat Heart Tissue Lysate at 40ug. Lane 2: Rat Brain Tissue Lysate at 40ug. Lane 3: Rat Liver Tissue Lysate at 40ug. Lane 4: Rat Skeletal Muscle Tissue Lysate at 40ug. Lane 5: PANC Whole Cell Lysate at 40ug. Lane 6: HELA Whole Cell Lysate at 40ug. Lane 7: SKOV Whole Cell Lysate at 40ug. Lane 8: COLO320 Whole Cell Lysate at 40ug. Predicted bind size: 17KD. Observed bind size: 17KD.
NME1 / NM23 Antibody - Flow Cytometry analysis of Hela cells using anti-NME1 antibody. Overlay histogram showing Hela cells stained with anti-NME1 antibody (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-NME1 Antibody (1µg/10E6 cells) for 30 min at 20°C. DyLight®488 conjugated goat anti-rabbit IgG (5-10µg/10E6 cells) was used as secondary antibody for 30 minutes at 20°C. Isotype control antibody (Green line) was rabbit IgG (1µg/10E6 cells) used under the same conditions. Unlabelled sample (Red line) was also used as a control.
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Polyclonal Rabbit anti‑Human NME1 / NM23 Antibody (aa137‑152, IHC, WB) LS‑C312974

Polyclonal Rabbit anti‑Human NME1 / NM23 Antibody (aa137‑152, IHC, WB) LS‑C312974

Note: This antibody replaces LS-C388959
Antibody:
NME1 / NM23 Rabbit anti-Human Polyclonal (aa137-152) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified
Price
Catalog Number
$318
LS-C312974-10
Toll Free North America
206-374-1102
For Research Use Only

Overview

Antibody:
NME1 / NM23 Rabbit anti-Human Polyclonal (aa137-152) Antibody
Application:
IHC, IHC-P, ICC, WB
Reactivity:
Human, Mouse
Format:
Unconjugated, Unmodified

Specifications

Description
NM23 antibody LS-C312974 is an unconjugated rabbit polyclonal antibody to NM23 (NME1) (aa137-152) from human. It is reactive with human and mouse. Validated for ICC, IHC and WB.
Target
Human NME1 / NM23
Synonyms
NME1 | AWD | NDKA | NB | NDK A | NM23-H1 | NDP kinase A | NDPK-A | NDPKA | GAAD | Granzyme A-activated DNase | NBS | NM23
Host
Rabbit
Reactivity
Human, Mouse (tested or 100% immunogen sequence identity)
Clonality
Polyclonal
Conjugations
Unconjugated
Purification
Immunogen affinity purified
Modifications
Unmodified
Immunogen
A synthetic peptide corresponding to a sequence at the C-Terminus of human NM23A(137-152 aa EELVDYTSCAQNWIYE), different from the related mouse sequence by two amino acids and from rat sequence by one amino acid.
Epitope
aa137-152
Specificity
Isoform 1 is expressed in heart, brain, placenta, lung, liver, skeletal muscle, pancreas, spleen and thymus. Expressed in lung carcinoma cell lines but not in normal lung tissues. Isoform 2 is ubiquitously expressed and its expression is also related to tumor differentiation. Isoform 3 is ubiquitously expressed. .
Applications
  • IHC
  • IHC - Paraffin
  • ICC
  • Western blot
Performing IHC? See our complete line of Immunohistochemistry Reagents including antigen retrieval solutions, blocking agents ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
Presentation
Lyophilized from 0.2mg Na2HPO4, 5mg BSA, 0.9mg NaCl, 0.05mg Thimerosal, 0.05mg sodium azide.
Reconstitution
Add 0.2ml of distilled water will yield a concentration of 500µg/ml.
Storage
At -20°C for 1 year. After reconstitution, at 4°C for 1 month. It can also be aliquotted and stored frozen at -20°C for a longer time. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NME1 / NM23
P15531 NM_000269 NP_000260.1

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Request SDS/MSDS

To request an SDS/MSDS form for this product, please contact our Technical Support department at:

Technical.Support@LSBio.com

Requested From: United States
Date Requested: 8/31/2024