Target
Human NKX2-1 / Thyroid-Specific TF
Synonyms
NKX2-1 | BCH | Benign chorea | Homeobox protein Nkx-2.1 | NK-2 | NKX2.1 | NK-2 homolog A | Thyroid transcription factor 1 | NK2 homeobox 1 | TTF-1 | BHC | Thyroid nuclear factor 1 | TITF1 | NKX2A | TEBP
Reactivity
Human, Mouse, Rat
(tested or 100% immunogen sequence identity)
Conjugations
Unconjugated
Immunogen
6His fusion protein corresponding to full-length TTF-1 (Thyroid transcription factor 1, also known as Thyroid nuclear factor 1, Homeobox protein Nkx-2.1).
Specificity
Recognizes TTF-1, Mr 42kD. Additional non-specific proteins also detected in longer exposures. Species cross-reactivity: Rat, mouse and human.
Applications
- IHC
- Western blot
- Immunoprecipitation
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ABC Detection Kits and polymers, biotinylated secondary antibodies, substrates and more.
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Usage
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Western Blot Analysis: A 1:500-1:1000 dilution of this lot detected TTF-1 in nuclear extract from H441 human lung epithelial cells. Immunoprecipitation: Has been reported by an independent laboratory to immunoprecipitate TTF-1. Immunohistochemistry: Has been reported by an independent laboratory to detect TTF-1 in 4% paraformaldehyde fixed human and mouse lung adenocarcinoma tissue.
Presentation
0.05% Sodium Azide, 30% Glycerol
Storage
Short term: store at 4°C. Long term: store at -20°C. Avoid freeze-thaw cycles.
Restrictions
For research use only. Intended for use by laboratory professionals.
Guarantee
This antibody carries the LSBio 100% Guarantee.
LSBio Guarantee
About NKX2-1 / Thyroid-Specific TF