Human NFYB

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography

Unmodified

NF-Y (B subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

N-Terminus

• Western blot (1:500 - 1:3000)
• ELISA (1:5000 - 1:25000)
• Gel shift

Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the 25 kD B subunit of NF-Y at a dilution of 1:500 followed by reaction with Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. Anti-NF-Y (13 subunit specific) is suitable for the detection by immunoblot of human and mouse NF-Y (13 subunit specific). Minimal reaction was observed by immunoblot against the 35 kD A subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 to 4.0 ul per assay. This product was assayed against NF-Y B subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti- Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:4000 is suggested from this experiment. Minimal reactivity was observed by ELISA against the A subunit of NF-Y.

0.01 M Sodium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Store vial at -20°C or below prior to opening. Dilute only prior to immediate use. Aliquot contents and freeze at -20°C or below. Avoid freeze-thaw cycles.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P25208 NCBI: NM_006166 NP_006157.1