Human NFYA

Rabbit

Human (tested or 100% immunogen sequence identity)

IgG Polyclonal

Unconjugated

Delipidation, salt fractionation and ion exchange chromatography

Unmodified

Anti-NF-Y (A) antibody was produced by repeated immunizations with a synthetic NF-Y (A subunit) peptide corresponding to a region near the N-terminus of the human protein conjugated to Keyhole Limpet Hemocyanin (KLH).

N-Terminus

• IHC
• IHC - Paraffin (5 µg/ml)
• Western blot (1:1000)
• ELISA (1:10000)

Suitable for immunoprecipitation, immunoblotting, ELISA and supershift assays. This product was assayed by immunoblot and found to be reactive against the differentially spliced 35 and 42 kD forms of the A subunit of human and mouse NF-Y at a dilution of 1:1000 followed by reaction with Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) catalog no. LS-C60884. No reaction was observed by immunoblot against the 25 kD B subunit of NF-Y. This product was also tested in a gel supershift assay and found to be reactive against human and mouse NF-Y using 2.0 ul per assay. This product was assayed against NF-Y A subunit peptide in an antibody capture ELISA using (#LS-C60884) Peroxidase conjugated Affinity Purified anti-Rabbit IgG [H&L] (Goat) and ABTS). A dilution of 1:10000 is suggested from this experiment. No reactivity was observed by ELISA against the B subunit of NF-Y.

0.02 M Potassium Phosphate, pH 7.2, 0.15 M NaCl, 0.01% Sodium Azide

Short term: -20°C; Long term: -70°C.

For research use only. Intended for use by laboratory professionals.

This antibody carries the LSBio 100% Guarantee.

Uniprot: P23511 NCBI: NM_021705 NP_068351.1